陕西医学杂志2017，Vol.46Issue(4)：414-416,3.DOI:10.3969/j.issn.1000-7377.2017.04.002
粪便SPG20、FBN1及VIM基因启动子甲基化联合检测在结直肠癌诊断中的意义
Detection of hypermethylated SPG20, FBN1 and VIM in stool samples of patients with colorectal cancer
摘要
Abstract
Objective: To clarify the feasibility and clinical significance of detection of SPG20, FBN1 and VIM gene promoter hypermethylation in stool samples of patients with colorectal cancer.Methods: Samples were collected from 75 patients with colorectal cancer and 30 healthy individuals in the first affiliate hospital of Xi'an Jiaotong University.The methylation status of SPG20, FBN1 and VIM gene promoter in tissue and stool samples was detected using methylation-specific PCR.Results: The methylation rates of SPG20, FBN1 and VIM gene promoter in cancer tissue samples were significantly higher than the distal tissue samples in patients with colorectal cancer.Also, the methylation rates of SPG20, FBN1 and VIM gene promoter in stool samples of patients with colorectal cancer were significantly higher than the healthy individuals.The sensitivity and specificity of combined detection of SPG20, FBN1 and VIM gene promoter methylation status in stool samples in detecting colorectal cancer were 96.0% and 86.7%, respectively.Conclusions: Multi-targets detection of SPG20, FBN1 and VIM gene promoter methylation status in stool samples is a promising approach in the screening of colorectal cancer.关键词
结直肠肿瘤/甲基化/@痉挛性截瘫-20/@微纤维蛋白-1Key words
Colorectal Neoplasms/Methylation/@SPG20/@FBN1分类
医药卫生引用本文复制引用
尹建浩,张昊,孟磊,许刚,周章建,张勇,党诚学..粪便SPG20、FBN1及VIM基因启动子甲基化联合检测在结直肠癌诊断中的意义[J].陕西医学杂志,2017,46(4):414-416,3.基金项目
国家自然科学基金资助项目(NSFC81372280) （NSFC81372280）
