实用妇产科杂志2017，Vol.33Issue(3)：189-193,5.
核转录因子-κB与妊娠期糖尿病发病机制的研究
Nuclear Transcription Factor-κ B and the Relationship between the Pathogenesis of Gestational Diabetes
摘要
Abstract
Objective:To regulate the nuclear transcription factor-κB(NF-κB) state in the cell signal transduction by investigating the relationship between NF-κB and gestational diabetes mellitus(GDM).Also,to investigate the development of inflammation TERMINA-LS and the role of NF-κR in GDM inflammatory pathogenesis.Methods:30 cases of normal pregnant women (W group) and GDM pregnant women (G group) were selected and measured the amount of LPS in serum.Samples in W group and G group were divided into four groups,labeled as W(G) group,W(G) + L group,W (G) + P group,W (G) + LP group respectively.W (G) group were treated with nothing,W(G) + L group were treated with LPS (1 μg/ml),W (G) + P group were treated with PDTC (50 mmol/L),W(G) + LP group were treated with PDTC(50 mmol/L) for 1 hour and then LPS(1 iμg/ml) was added.Western blot method was used to detect the actfvation of NF-κB protein in the cell while ELISA method was used to detect the expression levels of inflammatory markers (TNF-α,IL-1,IL-10) in the supernatant.Results:①The amount of LPS in serum of GDM pregnant women was higher than that of normal pregnant women(P＜ 0.05).②2Comparison within groups:After stimulated by LPS,the activation of NF-κB and the levels of TNF-α,IL-1 and IL-10 in W(G) + L group and W(G) + LP group were significantly higher than those in W(G) (P＜0.05);After added PDTC to block NF-κB activation,the activation of NF-κB and the levels of TNF-α,IL-1 and IL-10 in the W(G) +LP group were significantly lower than those in the control group W(G) + L group(P＜0.05).③Comparison between the two groups:The activation of NF-κB / p65 and the levels of TNF-α,IL-1 and IL-10 in the GDM group were significantly higher than those in the normal control group (P ＜ O.05).④Correlation analysis showed a positive correlation between the expression of NF-κB and inflammatory factor levels(P ＜ 0.05).Conclusions:From the results,we found that NF-κB plays a pivotal role in mediating several intracellular signaling pathways in the nucleus.NF-κB is involved in the occurrence of GDM by regulating the TERMINALS inflammatory cytokines during pregnancy.关键词
妊娠期糖尿病/终末炎症因子/核转录因子-κBKey words
Gestational diabetes mellitus/Inflammation/NF-κB分类
医药卫生引用本文复制引用
周培,丛林,袁静,方慧琴,陈薇,李琴,杨琴..核转录因子-κB与妊娠期糖尿病发病机制的研究[J].实用妇产科杂志,2017,33(3):189-193,5.基金项目
安徽省自然科学基金(编号:1208085MH1720) （编号:1208085MH1720）
