生殖医学杂志2017，Vol.26Issue(4)：308-312,5.DOI:10.3969/j.issn.1004-3845.2017.04.003
植入前遗传学筛查用于几种适应症的效率初探
Efficiency of preimplantation genetic screening in applying to different indications
孙贻娟 1曹英 1张硕 1伏静 1滕彬 1谷瑞环 1王宁怡 1孙晓溪1
作者信息
- 1. 复旦大学妇产科医院集爱遗传与不育诊疗中心,上海 200011
- 折叠
摘要
Abstract
Objective:To explore the efficiency of preimplantation genetic screening (PGS)in applying to the patients with chromosome abnormality,recurrent implantation failure (RIF),and recurrent spontaneous abortion (RSA).Methods:A total of 328 PGS treatment cycles and 263 subsequent frozen embryo transfer (FET) cycles in our reproductive center from Jan.2015 to Mar.2016 were included in this study.The patients were divided into four groups according to different indications:group A with patients of husband's chromosome abnormality,group B with patients of wife's chromosome abnormality,group C with patients of RIF,and group D with patients of RSA.The basic information,the clinical outcomes including in vitro fertilization and embryo development,single nucleotide polymorphism (SNP) results and clinical outcome of FET cycles were analyzed among the four groups.Results:There were no differences in oocyte MII rate,fertilization rate,cleavage rate,usable embryo rate on Day 3 among the four groups (P>0.05).The number of oocytes retrieved in group A (12.93±5.73) or in group B (13.17±6.65) were significantly higher than that in group C (10.48±6.07) or group D (10.76±5.47).The ratio of the number of blastocysts available for PGS and the number of usable embryos on Day 3 in group D (57.2%) was significantly higher than that in group A (51.3%),group B (48.4%) or group C (50.0%) (all P<0.05).The rates of normal karyotype embryo in group C (71.6%) and group D (58.7%) were significantly higher than that of group A (39.0%) and group B (33.5%).The rate of cycles with no normal karyotype embryos for transplantation in group D (13.5%) was significantly lower than that in group A (31.3%),group B (33.3%) or group C (29.1%) (all P<0.05).The clinical outcomes including clinical pregnancy rate and abortion rate were not significantly different among the four groups (P>0.05).Conclusions:The patients with the three different indications can get satisfactory clinical outcomes by PGS.The couples with chromosome abnormality have lower probability to obtain normal karyotype embryos,but are effective in avoiding the risk of high abortion rate.The abortion rate of patients with RSA can be controlled at a low level (3.4%) by means of PGS technique.关键词
植入前遗传学筛查/反复种植失败/复发性流产/单核苷酸多态性/冻融胚胎移植Key words
Preimplantation genetic screening/Recurrent implantation failure/Recurrent spontaneous abortion/Single nucleotide polymorphism/Frozen embryo transfer引用本文复制引用
孙贻娟,曹英,张硕,伏静,滕彬,谷瑞环,王宁怡,孙晓溪..植入前遗传学筛查用于几种适应症的效率初探[J].生殖医学杂志,2017,26(4):308-312,5.
