生殖医学杂志2017，Vol.26Issue(4)：313-319,7.DOI:10.3969/j.issn.1004-3845.2017.04.004
人卵泡壁层颗粒细胞源性非整合诱导多能干细胞的制备
Derivation of integration-free iPSCs from human mural granulosa cells
摘要
Abstract
Objective:To induce human mural granulose cells to integration-free induced pluripotent stem cells(iPSC),and test the differentiation potential of granulosa cell-like (GC-like) cells.Methods:Human mural granulosa cells were collected during oocyte retrieval process from women undergoing infertility treatment.iPS Sendai virus was added to the 4th day of granulosa cell cultured.After 20 days of maintenance,iPS cell clones were picked and cultured.The ability of pluripotent gene expression,the integration of foreign genes,and the ability of differentiation of GC-like cells were identified and compared with the iPSC derived from skin cells.Results:mGC-iPSCs were successfully generated,and passed the tests of similar pluripotency of embryonic stem cells and differentiation ability of three germ layers in vitro.The differentiated GC-like cells expressed the granulosa cell specific marker FOXL2,CYP19A1 and FSHR.These GC-like cells were also capable of producing AMH and aromatizing testosterone to estradiol,which suggested that they were biologically functional.mGC-iPSCs have higher differentiation` efficiency than (fi)broblast-iPSCs.Conclusions:The study provides a safety method for generating human iPSCs from human mural granulosa cells,and finds that mGC-derived iPSCs reveal higher differentiation efficiency than (fi)broblast-derived iPSCs.The system can not only be used to establish the iPSC library for reproductive infertility,but also to provide a new idea for cell therapy for the infertile patients with granule cell dysfunction.关键词
非整合诱导多能干细胞/壁层颗粒细胞/分化效率Key words
Integration-free iPSCs/Mural granulosa cells/Differentiation efficiency引用本文复制引用
蔡炳,脱颖,李宇彬,麦庆云,刘新颜,徐艳文,周灿权..人卵泡壁层颗粒细胞源性非整合诱导多能干细胞的制备[J].生殖医学杂志,2017,26(4):313-319,7.基金项目
国家自然青年基金(31401269) （31401269）
广东省公益研究基金(2014A020211012) （2014A020211012）
广州市生殖医学转化中心项目(201508020006) （201508020006）
