生殖医学杂志2017，Vol.26Issue(4)：362-365,4.DOI:10.3969/j.issn.1004-3845.2017.04.014
PRM1基因C321A、G197T单核苷酸多态性对体外受精结局的影响
Association between C321A & G197T polymorphism of PRM1 gene and IVF fertilization rate
李伟伟 1闫娅妮 1史鸿志 1董丽霞 1殷秀荣1
作者信息
- 1. 秦皇岛市妇幼保健院生殖医学科,秦皇岛 066004
- 折叠
摘要
Abstract
Objective:To explore the association between single nucleotide polymorphism (SNP) loci C321A & G197T in protamine 1 (PRM1) gene and IVF fertilization rate.Methods:A total of 182 IVF-ET cycles in our reproductive center were collected.The cycles were divided into three groups by the fertilization rate:the cycles with fertilization rate<30% in group A (n=43),30% to 65% in group B (n=24) cycles,and>65% in group C (n=115).The genomic DNA of residual sperm after insemination was extracted,and the frequency of C321A and G197T in PRM1 was detected by PCR-RFLP analysis.The differences in genotype and IVF fertilization rate were compared among the three groups.Results:There was no any significant difference in the genotype frequency of C321 polymorphism in PRM1 gene among the three groups(P>0.05).C321A polymorphism in PRM1 gene was not associated with IVF fertilization rate(P>0.05).The frequency of genotype GG,GT and TT of G197T in PRM1 gene was 9.3%(4),25.6%(11),and 65.1%(28)in group A;the frequency of genotype GG,GT and TT was 8.3%(2),25.0%(6),and 66.7%(16)in group B;and the frequency of genotype GG,GT and TT was 38.3%(44),26.1%(30),and 35.6%(41)in group C respectively.The frequency of genotype TT of G197T in group A and group B was significantly higher than that of group C(65.1% vs.35.6%,66.7% vs.35.6%)(P<0.05).Genotype TT of PRM1 G197T increased the risk of fertilization failure.Conclusions:The SNP of C321A in PRM1 is not associated with IVF fertilization rate.The SNP of G197T in PRM1 is associated with IVF fertilization rate.关键词
鱼精蛋白1/单核苷酸多态性/体外受精Key words
Protamine 1/Single nucleotide polymorphism/IVF引用本文复制引用
李伟伟,闫娅妮,史鸿志,董丽霞,殷秀荣..PRM1基因C321A、G197T单核苷酸多态性对体外受精结局的影响[J].生殖医学杂志,2017,26(4):362-365,4.
