生殖医学杂志2017，Vol.26Issue(4)：366-371,6.DOI:10.3969/j.issn.1004-3845.2017.04.015
人脐带间充质干细胞移植对大鼠流产模型妊娠结局的影响
Effect of transplantation of Wharton jelly-derived mesenchymal stem cells on pregnancy outcome in rats of abortion model
摘要
Abstract
Objective:To investigate the effect of transplantation of Wharton jelly-derived mesenchymal stem cells (WJ-MSCs) on the pregnancy outcome in the rats of abortion model.Methods:Forty eight pregnancy rats were divided into six groups and eight for each group: the rats were given subcutaneous injection of saline (0.4 mg·kg-1·d-1) from the 6th to 8th of pregnancy,and tail intravenous injection of saline (1 ml/kg) on the 9th day of pregnancy in saline control group (group a);the rats were given subcutaneous injection of 75% ethanol (0.4 mg·kg-1·d-1) from the 6th to 8th of pregnancy,and tail intravenous injection of saline (1 ml/kg) on the 9th day in ethanol control group (group b);the rats were given subcutaneous injection of bromocriptine (0.4 mg·kg-1·d-1) from the 6th to 8th of pregnancy,and tail intravenous injection of saline (1 ml/kg) on the 9th day in miscarriage model group (group c);the rats given subcutaneous injection of bromocriptine (0.4 mg·kg-1·d-1) from the 6th to 8th of pregnancy,and tail intravenous injection of 1×105,1×106 and 1×107 WJ-MSCs respectively in group d1,d2 and d3 respectively.All the rats were killed and collected the tissue materials at the 14th gestation days.The embryo resorption rate was observed in every group.The mRNA expression of interleukin (IL)-10,interferon-γ (IFN-γ) and IL-17 in the decidual tissues were detected by RT-PCR.The levels of IL-10,IFN-γ and IL-17 in peripheral blood were detected by ELISA.Results:Embryos absorption rate in group c and group d1 was significantly higher than that in group a,b,d2 & d3 (P<0.05).The mRNA expression of IL-10 was significantly decreased,but mRNA expressions of IFN-γ和IL-17 were significantly increased in group C & d1 compared with group a,b,d2 & d3 (P<0.05).The IL-10 level was significantly decreased and IFN-γ & IL-17 levels were significantly increased in peripheral blood in group c & d1 compared with group a,b,d2 & d3 (P<0.05).There were no significant differences in the observation index between group c and d1,group a,b and group d2,d3 (P>0.05).Conclusions:Transplanting certain amount of WJ-MSCs into the rats of abortion model may reduce the embryo absorptivity,and improve the outcome of pregnancy.The mechanism maybe related with WJ-MSCs up-regulating the expression of IL-10 and down-regulating the expression of IFN-γ and IL-17.关键词
流产/大鼠/Th1/Th2/Th17细胞亚群/人脐带间充质干细胞Key words
Abortion/Rat/Th1/Th2/Th17 cell subsets/Wharton jelly-derived mesenchymal stem cells引用本文复制引用
陈肖静,杨晓清,陈薇薇,吴荣荣,谢绘绘,张玉泉..人脐带间充质干细胞移植对大鼠流产模型妊娠结局的影响[J].生殖医学杂志,2017,26(4):366-371,6.基金项目
国家青年自然科学基金资助项目(81401272) （81401272）
江苏省妇幼保健重点资助项目(F201312) （F201312）
