特产研究2017，Vol.39Issue(1)：22-25,35,5.DOI:10.16720/j.cnki.tcyj.2017.01.005
UPLC-ELSD法测定人参中果糖、葡萄糖、蔗糖、麦芽糖含量
UPLC-ELSD Simultaneous Determination of Fructose,Glucose,Sucrose and Maltose in Ginseng
摘要
Abstract
A method for the simultaneous determination of 4 sugars such as fructose,glucose,sucrose and maltose in ginseng is described,in which ultra performance liquid chromatography and evaporative light scattering detectionare used.A acquity uplc BEH Amide (2.1 mm× 100mm,1.7μm)column kept at 35 ℃ and eluted with 0.2％ TEA acetonitrile solution and aqueous at a flow rate of 0.2mL/min was used for the chromatographic separation.The detector drift tube was kept at 75℃ and the nitrogen pressure was set at 30 psi.The response of the detector to analyte concentration over the range from 0.1 mg/mL to 0.8mg/mL displayed good linearity.Precision RSDs of 6 replicates for the determination of the sugars were less than 5.0％ and mean recoveries for the sugars in 6 real samples were more than 96.0％.A good separation among the analytes and other components was achieved within 7 min.This method is accurate and good reproducibility suitable for the measurement of four sugars in the roots of ginseng.关键词
超高效液相色谱法/蒸发光散射检测/人参/糖Key words
UPLC/ELSD/Panax ginseng C.A.Mey./sugar分类
医药卫生引用本文复制引用
逄世峰,李亚丽,许世泉,郑培和,崔丽丽,王英平..UPLC-ELSD法测定人参中果糖、葡萄糖、蔗糖、麦芽糖含量[J].特产研究,2017,39(1):22-25,35,5.基金项目
公益性行业(农业)科研专项(201303111) （农业）
国际科技合作专项(2015DFA31290) （2015DFA31290）
吉林省重点专项(20150204052YY) （20150204052YY）
