天津中医药2017，Vol.34Issue(4)：266-271,6.DOI:10.11656/j.issn.1672-1519.2017.04.16
HPLC-MS/MS法同时测定大鼠血浆中山楂叶提取物3种黄酮类成分及其药代动力学研究
Simultaneous determination of three flavonoids in hawthorn leaves extract by HPLC-MS/MS method and analysis of their pharmacokinetics in plasma of rats
摘要
Abstract
[Objective] To establish a HPLC-MS/MS method for the stimultancous pharmacokinetic study of vitexin-4"-O-glucoside(VOG), vitexin-2"-O-rhamnoside(VOR) and vitexin(VIT) in rat plasma after administration of hawthorn leaves extract orally. [Methods] Samples of plasma were obtained at different time after rats were administrated with three doses of hawthorn leaves extract (1, 2, 4 g/kg). After the deproteinization by methanol, extract of plasma samples were separated on a Agilent ZORBAX XDB-C18 column (3.5μm,2.1×50 mm) with gradient elution using methanol and water (containing 0.1%formic acid) as mobile phase. Mass spectrometric detection was carried out by multiple reaction monitoring (MRM) using electrospray ionization in negative ion mode. [Results] A good linearity of VIT, VOG and VOR was shown in the ranges of 0.50~500.0 ng/mL (r=0.998), 1.0~1000.0 ng/mL(r=0.999) and 1.0~1000.0 ng/mL(r=0.998), respectively. The methods of precision, accuracy, recovery and stability are in line with the requirements of determination of biological samples. [Conclusion] The method is proved to be simple, rapid, and specific, and to be suitable for the simultaneous determination of VIT, VOG and VOR in plasma of rats and the pharmacokinetic study.关键词
药代动力学/高效液相色谱质谱联用法/山楂叶提取物Key words
pharmacokinetics/HPLC-MS/MS/hawthorn leaves extract分类
医药卫生引用本文复制引用
马浩玲,张德芹,丁丽琴,陈新则,吴晓磊,伍明江,邱峰..HPLC-MS/MS法同时测定大鼠血浆中山楂叶提取物3种黄酮类成分及其药代动力学研究[J].天津中医药,2017,34(4):266-271,6.基金项目
国家自然科学基金重点项目(81430095) （81430095）
国家重大新药创制科技重大专项(2012ZX09101212). （2012ZX09101212）
