天然产物研究与开发2017，Vol.29Issue(2)：217-223,7.DOI:10.16333/j.1001-6880.2017.2.006
绞股蓝总苷对高脂血症大鼠血管平滑肌细胞表型的影响
Effects of Gypenosiedes on Vascular Smooth Muscle Cells Phenotype in Hyperlipidemic Rats
摘要
Abstract
The aim of this study was to investigate the functions and regulatory pathway of gypenosiedes (GP) on vascular smooth muscle cells (VSMCs) phenotype in hyperlipidemic rats.Hyperlipidemic rats model were established by feeding rats with high-fat diet.Blood lipid levels were detected in all groups.The ultrastructure of VSMCs was detected by transmission electron microscope.The expression of smooth muscle actin (SM-actin),proliferating cell nuclear antigen (PCNA) and monocyte chemotactic protein-1 induced protein (MCPIP1) in VSMCs were determined by using immunohistochemistry staining and Western blot methods.The experimental results showed that GP can decrease the blood lipids levels.GP effectively attenuated dedifferentiated changes of VMSCs ultrastructure and increased the expression of SM-actin.The expressions of PCNA and MCPIP1 in VSMCs were both decreased in GP group.These results demonstrate a novel protection role of GP in VSMCs dedifferentiation by inhibiting MCPIP1 expression.关键词
绞股蓝总苷/血管平滑肌细胞/表型/单核细胞趋化蛋白-1诱导蛋白1Key words
gypenosides/vascular smooth muscle cell/phenotype/monocyte chemotactic protein-1 induced protein 1分类
医药卫生引用本文复制引用
束波,刘志江,钱民章..绞股蓝总苷对高脂血症大鼠血管平滑肌细胞表型的影响[J].天然产物研究与开发,2017,29(2):217-223,7.基金项目
贵州省中医药管理局中医药、民族医药科学技术研究课题(QZYY-2015-152) （QZYY-2015-152）
遵义医学院2012招标课题(F-613) （F-613）
