天然产物研究与开发2017，Vol.29Issue(2)：224-228,211,6.DOI:10.16333/j.1001-6880.2017.2.007
紫堇灵对四氯化碳致小鼠急性肝损伤的保护作用及机制的研究
Hepatoprotective Effect of Corynoline on Carbon Tetrachloride-induced Hepatotoxicity in Mice
摘要
Abstract
To investigate the hepatoprotective activities of Corynoline in the mice model of carbon tetrachloride (CCl4)-induced liver toxicity.In addition,attempts were made to elucidate the possible mechanism of action.Hepatotoxicity was induced in Kunming mice by a intraperitoneal injection (i.p.) of CCl4,10 mL/kg body weight,diluted with corn oil at a ratio of 1:500.The corynoline (COR) was administered once a day for 7 days (i.p.) as pretreatment at 10 mg/kg ·day.The levels of C-reactive protein (CRP) and tumor necrosis factor-α (TNF-α) were analyzed to determine the inflammation status.The levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were analyzed by ELISA.Liver ultrastructure was observed via optical microscopy.The protein expression degrees of peroxisome proliferator-activated receptor gamma (PPARγ) and nuclear factor-k-gene binding (NF-κB) were assayed by Western blot.CC14-induced hepatotoxicity was manifested by an increase in the levels of ALT,AST,CRP and TNF-α.The histopathological examination of liver sections revealed necrosis and inflammatory reactions.The pretreatment with COR decreased levels of ALT,AST,CRP and TNF-α,decreased the protein expression degrees of NF-κB,and the protein expression degrees of PPARγ,and normalized the hepatic histo-architecture.This study supported the use of COR against hepatotoxicity,and the hepatoprotective effect was mainly through PPARγ and NF-κB signaling passway.关键词
紫堇灵/四氯化碳/急性肝损伤/抗炎/过氧化物酶增殖物激活受体γKey words
Corynoline/carbon tetrachloride/anti-inflammatory/hepatotoxicity/peroxisome proliferator-activated receptor分类
医药卫生引用本文复制引用
冯培文,韩吉春,李德芳,王凤华,房雪,郑秋生..紫堇灵对四氯化碳致小鼠急性肝损伤的保护作用及机制的研究[J].天然产物研究与开发,2017,29(2):224-228,211,6.基金项目
国家自然科学基金(31471338) （31471338）
山东省高等学校优势学科人才团队培育计划 （）
滨州医学院科研启动基金(BY2014KYQD01) （BY2014KYQD01）
新疆特种植物药物质基础与作用机制研究创新团队支持项目(2015BD005) （2015BD005）
