天然产物研究与开发2017，Vol.29Issue(2)：229-234,6.DOI:10.16333/j.1001-6880.2017.2.008
迷迭香提取物对小鼠急性酒精肝模型保护作用研究
Protective Effect of Rosemary Extract on Acute Alcoholic Liver Injury in Mice
摘要
Abstract
To study the protection of Rosemary Extract (RE) on acute alcoholic liver injury in mice,and research the mechanism from alcohol metabolism,lipid metabolism,anti-oxidative and anti-inflammation.Mice were randomly divided into control group,model group,Metadoxine Capsules group (MC,200mg/kg · d),RE group (80,160,400 mg/kg · d).The mice were given drugs by gavage 1 time a day and for 30 days.Then,acute alcoholic liver injury model was established by intragastric administration of 56％ alcohol (13mg/kg) to the medication and model groups 1 h later after the last administration.12h later,the levels of serum ALT,AST,TG,TNF-α,LI-6,IL-10 were detected.The contents of hepatic MDA,SOD,GSH-Px,ADH were determined.The mRNA levels of FAS,ADRP,CYP2E1,PPARα and Caspase3 in liver were also measured by real time PCR.HE straining was performed for observing pathological changes of the liver tissues.Compare with model group,RE can reduce the activity of ADH and contents of ALT,AST,and TG in serum,and shorten the sober time,down regulate Caspase3 expression and relieve acute alcohol liver tissue lesions.The protection mechanism research found thatRE can suppress acute ALD by down-regulatingFAS and ADRP,which can reduce the TG concentration in serum.RE also cure acute ALD by enhancing the activity of SOD,GSH-Px and expressing of PPARα,and down-regulating the expression of CYP2E1,to reduce the level of MDA in liver and relieve oxidative damage.RE can reduce the hepatic inflammation by decreasingTNF-α,IL-6 and increasing IL-10.These results showed thatRE can protect acute alcoholic liver injury by anti-oxidative,anti-inflammation and regulating lipid metabolism.关键词
迷迭香提取物/急性酒精肝/氧化损伤/脂代谢/炎症Key words
rosemary extract/acute alcoholic liver injury/oxidative damage/lipid metabolism/inflammation分类
医药卫生引用本文复制引用
张志毕,杨晖,张媛,于浩飞,胡炜彦,张荣平..迷迭香提取物对小鼠急性酒精肝模型保护作用研究[J].天然产物研究与开发,2017,29(2):229-234,6.基金项目
云南省南药研究协同创新中心项目(NY2014002) （NY2014002）
