天然产物研究与开发2017，Vol.29Issue(2)：278-283,6.DOI:10.16333/j.1001-6880.2017.2.017
基于UPLC-MS/MS法研究淫羊藿属植物品种品质与生长海拔、植物类群间关系
Correlations Between Quality and Species, Altitude,Taxa of Epimedium Plants by UPLC-MS/MS
摘要
Abstract
In this study,the content of the main components in Epimedium was compared by UPLC-MS/MS analysis to explore the correlation between Epimedium quality,growth altitude and plant groups.The analysis was performed on a Phenomenex-C18 column (50 mm × 2.1 mm,3 μm).The mobile phase consisted of acetonitrile (B) and 0.1％ formic acid (A) were used for gradient elution.The flow rate was 0.2 mL/min.The ion source of MS were ESI with multiple reaction monitoring mode.A good linear relationship between icariin,epidemin A,epidemin B and epidemin C were found in the concentration range of 0.995-9950.000,0.992-9920.000,1.021-10210.000 and 1.035-10350.000 ng/mL,respectively.The average recovery rate were 98.7％-101.2％ and the RSD were 1.7％-2.4％.The result showed that the content of main components were significantly different in different species of Epimedium,and the content decreased with the increasing of elevation.The content of each components in small-flowered taxa of Epimedium were significantly higher than the small-flowered taxa.This methods were accurate,rapid,highly sensitive and suitable for the qualitative analysis of main component of Epimedium.关键词
淫羊藿/UPLC-MS/MS/海拔高度/植物类群/质量分析Key words
Epimedium/UPLC-MS/MS/altitude/plant groups/quality analysis分类
医药卫生引用本文复制引用
秦伟瀚,阳勇,刘翔,张小梅,赵纪峰,郭延垒..基于UPLC-MS/MS法研究淫羊藿属植物品种品质与生长海拔、植物类群间关系[J].天然产物研究与开发,2017,29(2):278-283,6.基金项目
国家重大新药创制重大新药专项(2013ZX0101108) （2013ZX0101108）
重庆市科委前沿与应用基础研究(estc2014jcyjA10128) （estc2014jcyjA10128）
重庆市科委基本科研业务(2015cstc-jbky-01902) （2015cstc-jbky-01902）
重庆市卫生局中医药科技项目(ZY20132071) （ZY20132071）
