西北国防医学杂志2017，Vol.38Issue(3)：148-151,4.DOI:10.16021/j.cnki.1007-8622.2017.03.003
Notch3过表达对心肌成纤维细胞向肌成纤维细胞转化的抑制作用
Inhibits effect of overexpression of Notch3 in transformation of cardiac fibroblasts to fibroblasts
摘要
Abstract
Objective:To investigate the role of overexpression of Notch3 in the transformation of cardiac fibroblasts (CFs) to myofibroblasts (MFs).Methods:CFs were isolated by collagenase digestion method and incubated for 24 h with 10 ng/ml TGF-β1.Hydroxyproline kit was used to measure hydroxyproline content in the supernatants,immunofluorescence was used to measure α-smooth muscle actin (αSMA) expression,western blotting analysis was used to measure Notch3 expression.Lentiviral was used to transfect CFs,overexpression of Notch3.When the multiplicity of infection (MOI) was 20,transfected into 72 h was the optimal transfection condition,fluorescence microscopy was used to observe the expression of green fluorescent protein (GFP),western blotting analysis was used to measure the efficiency of Notch3 overexpression.Hydroxyproline kit was used to measure hydroxyproline content in the supernatants,immunofluorescence was used to measure α-SMA expression.Results:The 10 ng/ml TGF-β1 induced increases of hydroxyproline content in the supernatants and α-SMA expression significantly in CFs and induced decreases of Notch3 expression.Compared with the blank control group,there were significant differences (P＜0.05).Lentiviral transfection of Notch3 overexpression,compared with the blank control group,hydroxyproline content in the supernatants and α-SMA expression decreased in overexpression group (P ＜ 0.05).Conclusion:overexpression of Notch3 can inhibit the transformation of cardiac fibroblasts to myofibroblasts.关键词
心肌成纤维细胞/Notch3/过表达/肌成纤维细胞/转化Key words
cardiac fibroblasts/Notch3/overexpression/myofibroblasts/transformation分类
医药卫生引用本文复制引用
黄玉霞,姚茹,邹倩,范延红..Notch3过表达对心肌成纤维细胞向肌成纤维细胞转化的抑制作用[J].西北国防医学杂志,2017,38(3):148-151,4.基金项目
国家自然科学基金资助项目(81370194) （81370194）
