西北师范大学学报（自然科学版）2017，Vol.53Issue(2)：70-74,5.DOI:10.16783/j.cnki.nwnuz.2017.02.013
基于纳米金表面等离子体共振光散射法测定视黄醇结合蛋白
Plasmon resonance light scattering spectral assayof retinol binding protein based on gold nanoparticles
摘要
Abstract
Gold spheres of 12 nm prepared by reduction of sodium citrate are attached to retinol binding protein(anti-RBP).In pH=5.29 KH2PO4-Na2HPO4 buffer,the immune reaction between gold-labeled anti-RBP and retinol binding protein(RBP) is carried out,and the resonance light scattering intensity of 620 nm is increased significantly.ΔI620nm has a good linear relationship with concentration of RBP in 0.01-60 ng·mL-1,and the detection limits of RBP is 0.02 ng·mL-1.So it is a simple and sensitive method for the quantitative detecting of RBP in the clinical diagnosis with good results.关键词
纳米金/视黄醇结合蛋白/金标记/等离子体共振光散射Key words
gold nanoparticles/retinol binding protein/gold labeling/plasmon resonance light scattering分类
化学化工引用本文复制引用
邹明静,王淑玉,王英玲,李刚,刘雪莹..基于纳米金表面等离子体共振光散射法测定视黄醇结合蛋白[J].西北师范大学学报（自然科学版）,2017,53(2):70-74,5.基金项目
山东省高等学校科技计划资助项目(J11LB01) （J11LB01）
菏泽医学专科学校科研资助项目(H14k05) （H14k05）
