中国组织工程研究2017，Vol.21Issue(10)：1514-1519,6.DOI:10.3969/j.issn.2095-4344.2017.04.007
人β-防御素3/聚乳酸-羟基乙酸缓释微球制备及体外释药性能
Human beta defense 3/poly(lactic-co-glycolic acid) controlled-release microspheres preparation and in vitro release profile
摘要
Abstract
BACKGROUND: A simple use of antibiotic drugs as anti-infection therapy after joint replacement is not enough for subsequent debridement and secondary revision surgeries. Therefore, our team intended to confirm the feasible use of controlled-release microspheres in the local anti-infection treatment.OBJECTIVE: To prepare the Human beta defense 3 (HBD-3)/poly(lactic-co-glycolic acid) (PLGA) micro-spheres and to investigate the microsphere physicochemical properties and drug release profile in vitro.METHODS: With PLGA as a carrier,HBD-3/PLGA controlled-release microspheres were prepared by using double emulsion-solvent evaporation method. Scanning electron microscopy was used to observe its surface morphology.The size of each microsphere was accurately determined using scaleplate. Drug loading capacity and encapsulation efficiency of HBD-3/PLGA controlled-release microspheres were calculated using spectrophotometer. HBD-3/PLGA microsphere controlled-release time was determined in order to analyze the drug release profile of the microsphere. RESULTS AND CONCLUSION: The HBD-3/PLGA controlled-release microsphere possessed smooth surface, uniform distribution and good liquidity.The average particle size was 219.49 nm, the drug loading capacity of HBD-3 was (20.67±0.17)% and the encapsulation efficiency was (54.52±1.31)%. The cumulative release percentage of HBD-3 was(74.12±0.43)%. The HBD-3/PLGA controlled-release microsphere has well controlled-release performance in vitro. In theory, the purpose of antibacterial controlled-release can be achieved,laying a foundation for subsequent animal antibacterial experiments.关键词
生物材料/缓释材料/HBD-3/PLGA缓释微球/理化性质/载药量/包封率/体外释药性能分类
医药卫生引用本文复制引用
孙治邦,周义钦,陈松,吴海山..人β-防御素3/聚乳酸-羟基乙酸缓释微球制备及体外释药性能[J].中国组织工程研究,2017,21(10):1514-1519,6.基金项目
上海市卫生局课题(2012353),课题名称:β-防御素-3/聚乳酸聚乙醇酸缓释微球抑制人工关节术后生物膜形成的机制研究 Funding: the Project of Shanghai Health Bureau, No. 2012353 （2012353）
