中国组织工程研究2017，Vol.21Issue(10)：1520-1526,7.DOI:10.3969/j.issn.2095-4344.2017.10.008
载三联抗痨药硫酸钙/聚氨基酸缓释材料在兔脊柱结核模型体内的缓释性能
The slow release performance of calcium sulfate/poly(amino acid) compound materials carrying triple anti-tuberculosis drugs in a rabbit model of spinal tuberculosis
摘要
Abstract
BACKGROUND: Calcium sulfate/poly amino acid compound materials carrying triple anti-tuberculosis drugs have been proved to have excellent slow release performance based on our preliminary studies on the physical and chemical properties and the release properties of the compound materials.OBJECTIVE: To observe the slow release performance of the calcium sulfate/poly(amino acid) compound material carrying triple anti-tuberculosis drugs in a rabbit model of spinal tuberculosis.METHODS: Twenty-four New Zealand white rabbits were used to make L4-5 spinal tuberculosis models and divided into two groups in a random way following removal of tuberculosis lesions. Calcium sulfate/poly amino acid compound material carrying isoniazide, rifampicin, pyrazinamide or calcium sulfate/poly(amino acid)compound material with no drugs was implanted into the defect in the experimental or control group,respectively. At 2, 4, 6 and 8 weeks after implantation, the concentrations of isoniazid, rifampicin and pyrazinamide in the defect region, including the bone tissue, adjacent psoas major and inferior vena cava,were measured.RESULTS AND CONCLUSION: In the experimental group, the isoniazid levels in the damaged bone tissue and psoas major were kept in minimum bactericidal concentration (MBC) at 8 weeks after implantation and in the minimum inhibitory concentration (MIC) at the end of 12 weeks after implantation, while its level in the vein was kept in MBC at 2 weeks and in MIC at 8 weeks. The rifampicin levels in the bone tissue and psoas major were kept in MBC at 4 weeks after implantation and in the MIC at 8 weeks after implantation, while its level in the vein was kept MIC at 4 weeks.The pyrazinamide levels in the damaged bone tissue and psoas major were kept in MBC at 8 weeks after implantation and in the MIC until 8 weeks after implantation, while its level in the vein was kept MIC at 8 weeks. In the control group,there were no levels of isoniazid, rifampicin and pyrazinamide in the damaged bone tissue, adjacent psoas major and inferior vena cava in comparison with the baseline. These results show that isoniazid, rifampicin and pyrazinamide in the defect region can achieve sustained slow release in the rabbit model of spinal tuberculosis after implantation of the calcium sulfate/poly(amino acid) compound material carrying triple anti-tuberculosis drugs. In addition, the local drug concentration and duration in the defect region are better than those in the blood.关键词
生物材料/缓释材料/硫酸钙/聚氨基酸人工材料/异烟肼、利福平、吡嗪酰胺/局部缓释/高效液相色谱法/国家自然科学基金分类
医药卫生引用本文复制引用
王骞,耿广起,丛晓明,刘海涛,施建党,王自立,马文鑫,孙宇航..载三联抗痨药硫酸钙/聚氨基酸缓释材料在兔脊柱结核模型体内的缓释性能[J].中国组织工程研究,2017,21(10):1520-1526,7.基金项目
国家自然科学基金项目(81060149) （81060149）
宁夏自然科学基金项目(NZ10117) Funding: the National Natural Science Foundation of China, No. 81060149 （NZ10117）
the Natural Science Foundation of Ningxia Hui Autonomous Region, No. NZ10117 （）
