中国组织工程研究2017，Vol.21Issue(10)：1532-1538,7.DOI:10.3969/j.issn.2095-4344.2017.10.010
鱼皮胶原纤维重组构建纳吸棉的工艺特征
Optimization of fiber reconstituted technology for preparation of nasopore using fish scale collagen
摘要
Abstract
BACKGROUND: Nowadays, fish collagen biomedical materials still exhibit obvious deficiency in thermal stability,in vivo degradation stability and in vivo material morphology stability. To expand the application of fish source collagen, it is urgent to improve the material performance by increasing the density and collagen molecule tightness of freshwater fish collagen sponge materials using technique methods.OBJECTIVE: To optimize the reconstitute process for nasopore preparation using fish scale collagen.METHODS: The optimal process for nasopore preparation through the reconstitution of fish scale collagen was ascertained by taking tilapia fish skin as a raw material to extract enzymatic soluble collagen at a temperature lower than the collagen denaturation temperature and recombinant rate of collagen fibers as index. Optimization of the conditions for nasopore preparation was carried out using single factor test and orthogonal test. The prepared nasopore was analyzed through infrared spectroscopy and microstructure analysis.RESULTS AND CONCLUSION: The optimal conditions for nasopore preparation were determined through the single factor test and orthogonal test as follows: 20 ℃ for 10 hours at pH 7.4 using a mixture of 65 mmol/L NaCl and 1 g/L collagen, by which the reconstitute rate of collagen fibers was up to 68.6%. The prepared nasopore is characterized by a refined porous structure constituted by threadlike collagen fibers, and has complete three-dimensional spiral structure,which is a potential intracavitary hemostatic material with fine properties.关键词
生物材料/材料相容性/胶原/纤维重组/纳吸棉分类
医药卫生引用本文复制引用
李杰,刘东艳,秦松..鱼皮胶原纤维重组构建纳吸棉的工艺特征[J].中国组织工程研究,2017,21(10):1532-1538,7.基金项目
山东省科技发展计划 (2015GSF115032 ) Funding: the Science and Technology Development Plan of Shandong Province, No. 2015GSF115032 （2015GSF115032 ）
