中国组织工程研究2017，Vol.21Issue(10)：1546-1551,6.DOI:10.3969/j.issn.2095-4344.2017.10.012
新型鼻腔填塞材料聚乙二醇双丙烯酸酯复合物水凝胶的细胞相容性
Cytocompatibility of a novel nasal packing material made of polyethylene glycol diacrylate composite hydrogel
摘要
Abstract
BACKGROUND: Previous studies have shown that the complex of polyethylene glycol diacrylate and chitosan is biodegradable, has the anti-inflammation effect and can promote wound healing, with good biocompatibility.However, the composite material is brittle, and thus, it is necessary to develop a mechanical support material due to the anatomy of the nasal cavity.OBJECTIVE: To study the cytocompatibility of a novel degradable absorbable nasal packing material made of polyethylene glycol diacrylate composite hydrogel.METHODS: In our experiment, the composite hydrogel was formed by the combination of different ratios of polyethyleneglycol diacrylate, chitosan and polyvinyl alcohol using photo-crosslinking method. According to the international standard of medical devices, we used direct and indirect international standard experimental methods to evaluate the cytotoxicity of the materials. (1) Direct contact method: the polyethylene glycol acrylate composite hydrogel and mouse fibroblasts L929 were co-cultured for 24 hours to observe the morphological changes of cells. (2) Indirect extraction: mouse fibroblasts L929 were cultured in polyethylene glycol diacrylate composite hydrogel extracts (100, 50 μL) and RPMI1640 medium (100 μL), respectively, for 3 days to observe the proliferation and cytotoxicity by cell counting kit-8 assay.RESULTS AND CONCLUSION: (1) Direct contact method: the cells around the material formed a flat structure with fusiform or stellated protrudes. The material was confirmed to have good biocompatibility depending upon the presence of adherent cell growth, full and strong refraction, and the overall growth trend. (2) Indirect extraction method: with the increase of time, the number of cells in the hydrogel extract group increased, the relative proliferation rate of cells increased gradually, and the cytotoxicity was 0 to 1. To conclude, these experimental findings indicate that the polyethylene glycol acrylate composite hydrogel material has good cytocompatibility.关键词
生物材料/材料相容性/聚乙二醇双丙烯酸酯水凝胶/鼻填塞材料/细胞毒性/小鼠成纤维细胞分类
医药卫生引用本文复制引用
杨国慧,韩德民,臧洪瑞,李立峰..新型鼻腔填塞材料聚乙二醇双丙烯酸酯复合物水凝胶的细胞相容性[J].中国组织工程研究,2017,21(10):1546-1551,6.基金项目
北京市医院管理局"使命"人才计划项目(SML20150201) Funding: the Talent Plan Project of Beijing Municipal Administration of Hospitals, No. SML20150201 （SML20150201）
