中国组织工程研究2017，Vol.21Issue(11)：1664-1668,5.DOI:10.3969/j.issn.2095-4344.2017.11.005
前纵韧带损伤加重相应节段损伤椎间盘的退变
Degeneration of injured intervertebral disk affected by anterior longitudinal ligament destruction
摘要
Abstract
BACKGROUND: The spinal instability would accelerate the degeneration of normal disk. The injuries of anterior longitudinal ligament (ALL) and intervertebral disk were usually caused by cervical trauma, which leaded to spinal instability. Currently, there were few animal researches about the degeneration of injured intervertebral disk affected by ALL destruction.OBJECTIVE: To investigate the degeneration of injured intervertebral disk after spinal instability in the rabbit model of different degrees of ALL and disc destruction.METHODS: A total of 24 New-Zealand rabbits were randomly divided into intervertebral disk injury group (Group A, n=6), partial injury of ALL with disc injury group (Group B, n=6), injury of bony attachment point of ALL with disc injury group (Group C, n=6) and entirely injury of ALL with disc injury group (Group D, n=6). The L2-L3 intervertebral disk and ALL were injured through abdominal cavity. Different groups received different treatments. Computed tomography (CT) scans of the injured disc were performed at the postoperative 4 and 8 weeks, and the middle high of injured discs was calculated on CT sagittal reconstruction. Three rabbits were selected from each group. Hematoxylin-eosin staining of injured disc was performed after the animals were killed. Results were examined under the light microscope.RESULTS AND CONCLUSION: (1) At postoperative 4 weeks, the middle height of injured discs in Group D was decreased significantly compared with Group A (P < 0.05). There were hyperosteogeny and calcification in Group C and Group D. There were nucleus pulposus cells reduction and inflammatory reaction in Group C and Group D on histological staining. (2) At postoperative 8 weeks, the middle height of injured discs respective was decreased significantly compared with Group A (P < 0.05). The hyperosteogeny and calcification became clearer in Group C and Group D than before. There were morphologic changes of nucleus pulposus cells and fibrillation by hematoxylin-eosin staining, and the degree of disc degeneration was Group D > Group C > Group B > Group A. (3) In conclusion, the injury of ALL would accelerate the degeneration of correspondingly injured disk, and the degree of injury of ALL was positively correlated with the degeneration of disk.关键词
骨科植入物/脊柱植入物/前纵韧带/损伤/椎间盘/退变/脊柱失稳分类
医药卫生引用本文复制引用
孙鑫,金文杰,沈康平,刘兴振..前纵韧带损伤加重相应节段损伤椎间盘的退变[J].中国组织工程研究,2017,21(11):1664-1668,5.基金项目
上海交通大学医学院附属第九人民医院院基金(syz2015-024) a grant from Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, No.syz2015-024 （syz2015-024）
