中国组织工程研究2017，Vol.21Issue(12)：1872-1876,5.DOI:10.3969/j.issn.2095-4344.2017.12.012
佐剂性关节炎模型兔成纤维样滑膜细胞的培养
Culture of fibroblast-like synoviocytes from rabbits with adjuvant arthritis
王丽平 1刘琴 1陈芳 1陈利锋 2张宜1
作者信息
- 1. 中国人民解放军武汉总医院,医学实验科,湖北省武汉市 430070
- 2. 中国人民解放军武汉总医院,中西结合科,湖北省武汉市430070
- 折叠
摘要
Abstract
BACKGROUND:Currently, fibroblast-like synoviocytes from the synovial tissue of patients with rheumatoid arthritis or adjuvant arthritis rats are isolated and cultured mainly using enzyme digestion method, but tissue explant adherent method is rarely reported in primary culture. OBJECTIVE:To establish a culture method of fibroblast-like synoviocytes from adjuvant arthritis rabbits and to investigate cell biological characteristics. METHODS:Rabbit models of adjuvant arthritis were prepared by injected with adjuvant at multiple sites and times, the knee synovial tissues were obtained, and fibroblast-like synoviocytes were separated and cultured by the tissue explant adherent method. Furthermore, the cell morphology was observed. The cell proliferation was measured using cell counting kit-8 assay, and the expression of Vimentin was detected by immunofluorescence.RESULTS AND CONCLUSION:The primary cultured rabbit synoviocytes were long spindle fiber-like and coincided with the fibroblast-like synoviocytes. Moreover, cells were positive for Vimentin. In conclusion, fibroblast-like synoviocytes from rabbits with adjuvant arthritis are successfully isolated and cultured by the tissue explant method.关键词
组织构建/组织工程/佐剂性关节炎/兔/成纤维样滑膜细胞/组织块贴壁法分类
医药卫生引用本文复制引用
王丽平,刘琴,陈芳,陈利锋,张宜..佐剂性关节炎模型兔成纤维样滑膜细胞的培养[J].中国组织工程研究,2017,21(12):1872-1876,5.
