中国组织工程研究2017，Vol.21Issue(12)：1909-1914,6.DOI:10.3969/j.issn.2095-4344.2017.12.018
二甲双胍对2型糖尿病模型大鼠胰岛素抵抗的影响
Effect of metformin on the insulin resistance in a rat model of type 2 diabetes mellitus
秦凤 1李春亮 2张惠莉1
作者信息
- 1. 青海大学附属医院内分泌科,青海省西宁市 810001
- 2. 青海大学医学院,青海省西宁市 810016
- 折叠
摘要
Abstract
BACKGROUND:More than 10 kinds of lipid metabolism-related genes have been found, and endocrine dysfunction mediated by these genes is an important pathological basis for the occurrence and development of insulin resistance. OBJECTIVE:To investigate the effects of metformin on serum lipid profiles and the expression levels of various genes associated with insulin resistance, as well as the histopathological changes of the liver and pancreas in rats with type 2 diabetes melitus. METHODS:A type 2 diabetes mellitus rat model was established by feeding a high-fat diet to the rats for 4 weeks, combined with the intraperitoneal injection of streptozotocin (35 mg/kg). In the meanwhile, metformin was administered orally (400 mg/kg?d) (model group) or nothing (control group). Semi-quantitative RT-PCR, histopathological and biochemical examinations were then performed. RESULTS AND CONCLUSION:Metformin improved the symptoms of insulin resistance by normalizing the serum lipid profiles in the diabetic rats. Furthermore, metformin upregulated the expression levels of insulin receptors and genes associated with lipid metabolism, including acyl-CoA oxidase, carnitine palmitoyl transferase-1 and peroxisome proliferator activated receptor-α. In addition, metformin downregulated the expression levels of fetuin-A and retinol binding protein-4, and improved the expression of perilipin that had been reduced in the type 2 diabetes mellitus rats. Metformin was shown to induce positive signaling for insulin and the regeneration of pancreaticβcells in the pancreas. These results suggest that metformin ameliorates the insulin resistance induced by type 2 diabetes mellitus via regulating the expression levels of fetuin-A, retinol binding protein-4, perilipin and various genes associated with lipid metabolism.关键词
组织构建/组织工程/二甲双胍/2型糖尿病/胰岛素抵抗/机制分类
医药卫生引用本文复制引用
秦凤,李春亮,张惠莉..二甲双胍对2型糖尿病模型大鼠胰岛素抵抗的影响[J].中国组织工程研究,2017,21(12):1909-1914,6.
