厦门大学学报（自然科学版）2017，Vol.56Issue(2)：178-187,10.DOI:10.6043/j.issn.0438-0479.201604029
斜带石斑鱼干扰素调节因子8基因的鉴定与表达模式分析
Identification and Expression Pattern Analysis of Interferon Regulatory Factor 8 Gene in Orange Spotted Grouper,Epinephelus coioides
摘要
Abstract
Interferon regulatory factor 8 (IRF8) is a transcription factor in IRF family,and plays critical roles in the regulation of lineage commitment and in myeloidcell maturation including the decision for a common myeloid progenitor to differentiate into a monocyte precursor cell.In this study,an IRF8 gene was cloned from orange spotted grouper,Epinephelus coioides,using degenerate PCR and RACE.The full-length cDNA of EcIRF8 encodes 422 amino acid residues.The predicted peptide possesses two characteristic domains of IRF family,including a conserved DNA-binding domain with one nuclear localization signal and five conserved tryptophan residues in the N-terminal region,and a divergent C-terminal region that serves as the regulatory domain.EcIRF8 shares a nine-exon/eight-intron structure with its vertebrate homologues,with one intron in 5′-UTR.In normally cultured orange-spotted grouper,EcIRF8 was highly expressed in liver.In vivo,the transcriptional expression of EcIRF8 was significantly up-regulated in head kidney,middle kidney,thymus,liver and intestine,at 6 h post injection with Poly I∶C,and the maximum up-regulated expression of EcIRF8 was in middle kidney,which reached up to 106 folds compared with the control.In addition,a 9.3-fold increasing expression of EcIRF8 was found in liver at 24 h post injection with Vibrio parahemolyticus.The results suggested that EcIRF8 might be involved in both antiviral and antibacterial immune response of E.coioides,and mainly in defense from viral infection.关键词
干扰素调节因子8/斜带石斑鱼/活体聚肌胞苷酸Key words
interferon regulatory factor 8/Epinephelus coioides/Poly I∶C分类
生物科学引用本文复制引用
贾芹芹,黄贝,熊静,段利朋,徐继松,聂品,黄文树..斜带石斑鱼干扰素调节因子8基因的鉴定与表达模式分析[J].厦门大学学报（自然科学版）,2017,56(2):178-187,10.基金项目
国家自然科学基金(U205123,31172438,31402329) （U205123,31172438,31402329）
国家高技术研究发展计划(2012AA092201) （2012AA092201）
福建省自然科学基金(2012J06008,2014J05042) （2012J06008,2014J05042）
厦门南方海洋研究中心科技项目(14PYY050SF03) （14PYY050SF03）
