畜牧兽医学报2017，Vol.48Issue(3)：436-445,10.DOI:10.11843/j.issn.0366-6964.2017.03.006
四川麻鸭mtDNA全序列的克隆和生物信息学分析
Complete Sequence Cloning and Bioinformatics Analysis of Sichuan Sheldrake Duck Mitochondrial Genome
摘要
Abstract
The objective of this study was to obtain the complete mitochondrial DNA (mtDNA) sequence of Sichuan Sheldrake duck,and to provide reference data for protection and utilization of this genetic resources of duck.Sixteen pairs of primers were designed by Primer 6.0 according to the mitochondrial genome sequences of duck homologous species.The genomic sequence of Sichuan Sheldrake duck's mtDNA were then amplified by PCR and sequencing technology.The results showed that the length of Sichuan Sheldrake duck's mtDNA genome was 16 604 bp,which composed of A (29.19％),C (32.82％),G (15.79％) and T (22.20％).The whole genome contained 22 tRNA genes,2 rRNA genes,13 protein-encoding genes and a non-encoding control area (D-loop area).The analysis by online software of tRNAscan-SE 1.21 and RNA structure 5.6 found that 22 tRNA genes were the standard secondary structures with 4 constant arms which like a clover.The D-loop region of Sichuan Sheldrake duck's mtDNA genome contained Poly (c),TAS,E-box,F-box,D-box,Bird-similarity box and CXB1 conserved box.The neighbor joint (NJ) phylogenetic tree was established by MEGA6.0 basing on the D-loop region and the complete mtDNA sequence,respectively,and the results showed that the Longsheng duck was more closer to Sichuan Sheldrake duck.关键词
线粒体基因组/四川麻鸭/遗传进化Key words
mitochondrial genome/Sichuan Sheldrake duck/genetic evolution分类
农业科技引用本文复制引用
谢艾轩,吴启超,李思德,罗俊,张涛,刘贺贺,张军,何桦..四川麻鸭mtDNA全序列的克隆和生物信息学分析[J].畜牧兽医学报,2017,48(3):436-445,10.基金项目
四川省科技厅应用基础项目(2015JY0110) （2015JY0110）
大学生创新性试验项目(1510626046) （1510626046）
