畜牧兽医学报2017，Vol.48Issue(3)：446-453,8.DOI:10.11843/j.issn.0366-6964.2017.03.007
染色质重塑因子BPTF、BRG1在不同毛色绵羊皮肤的表达与定位
The Expression and Localization of Chromatin Remodeling Factor BPTF and BRG1 in Black and White Sheep Skin
摘要
Abstract
The objective of this study was to explore the expression and localization of chromatin remodeling factor BPTF and BRG1 in black and white sheep skin tissues.Skins were collected surgically from the hindquarters of 3 black sheep and 3 white sheep.The expression level and distribution of BPTF and BRG1 were detected by quantitative real-time-polymerase chain reaction (qRT-PCR),Western blotting,and immunohistochemistry to investigate the underlying mechanisms of coat color in white and black skin tissues of sheep,qRT-PCR and Western blotting results suggested that BPTF was expressed at significantly higher levels in black sheep compared with that of white sheep(P＜0.05);BRG1 was expressed at very significantly higher levels in black sheep compared with that in white sheep(P＜0.01).Immunohistochemistry demonstrated BPTF and BRG1 staining in the root sheath and dermal papilla in hair follicle of sheep skins.Therefore,it come to the conclusion that BPTF and BRG1 may involve in the coat color formation of sheep.关键词
BPTF/BRG1/绵羊/毛色Key words
BPTF/BRG1/sheep/coat color分类
农业科技引用本文复制引用
孙笑尉,张雪莲,李振,庞全海..染色质重塑因子BPTF、BRG1在不同毛色绵羊皮肤的表达与定位[J].畜牧兽医学报,2017,48(3):446-453,8.基金项目
国家自然科学基金(30972223 （）
31272628) （）
