畜牧兽医学报2017，Vol.48Issue(3)：454-461,8.DOI:10.11843/j.issn.0366-6964.2017.03.008
公山羊β防御素124的表达谱分析及其在繁殖器官中的定位
Expression Pattern Analysis of Goat Beta-defensin 124 and Its Location in the Reproductive Organs
摘要
Abstract
To study the expression pattern of goat beta-defensin 124 (gDB 124) in different organs and the location of gDB 124 in testis and epididymis of adult bucks.Testis,caput epididymidis,corpus epididymidis and cauda epididymidis were collected from 1-week-old male kids,2-6-monthold male kids and 18-month-old bucks.Part of the tissues were gathered from heart,liver,spleen,lung,kindey and longissimus dorsi of bucks at the age of 18 months.Analysis of gDB 124 mRNA expression pattern was detected by qRT-PCR.Location of gDB 124 in the reproductive organs was determined by immunofluorescence.The results of qRT-PCR showed that gDB 124 mRNA was detected in testis,caput,corpus,cauda,heart,liver,spleen,lung and kidney of bucks at the age of 18 months except the longissimus dorsi,and the expression level in caput of epididymis was extremely higher than that in other tissues (P＜0.01).Moreover,gDB 124 mRNA expression level in testis of buck at the age of 6 months was significantly higher than those at other ages.And the expressions in caput,corpus,cauda of epididymis were increased with the increase of the age.There was no obvious difference at gDB 124 mRNA expression levels between testis and epididymis of 1-week-old bucks.gDB 124 mRNA expressions in caput of epididymis were extremely higher than those in testis,corpus,cauda of epididymis of bucks at 3-6 and 18 months old (P＜0.05).The result of immunofluorescence suggested that gDB 124 protein was detected in testis and epididymis,and mainly located in false stratified columnar epithelium cells of caput of epididymis.The order of the expression level was caput ＞corpus＞cauda＞ testis,which was consistent with expression pattern of gDB 124 mRNA in testis and epididymis.In conclusion,the expression of gDB 124 was in the spatio-temporal expression.关键词
山羊/β防御素124/表达谱/定位/繁殖器官Key words
goat/beta-defensin 124/expression pattern/location/reproductive organs分类
农业科技引用本文复制引用
杜海燕,邰苗苗,姜玉锁,任有蛇,靳黎,乔利英,张春香..公山羊β防御素124的表达谱分析及其在繁殖器官中的定位[J].畜牧兽医学报,2017,48(3):454-461,8.基金项目
国家自然科学基金(31572407) （31572407）
