烟草科技Issue(12)：42-44,3.
应用点免疫结合法和组织印迹法检测烟草组织中的TMV和CMV
Detection of TMV and CMV in Tobacco Tissue by Dot-ELISA and Tissue Blot-ELISA
摘要
Abstract
Tobacco mosaic virus (TMV) and cucumber mosaic virus (CMV) were detected in tobacco tissue by dot-immunoblots enzyme-linked immunosorbent assay (ELISA) and tissue blot-ELISA.1 000-dilution rabbit-anti-TMV antibody could be used to detect purified TMV with limitation of 1.25ng/ml,and for TMV infected tobacco sap extracts with limitation of 1∶2 560.500-dilution rabbit-anti-CMV antibody could be used to detect CMV infected tobacco sap extracts with limitation of 1 ∶ 2 560.Detection of health tobacco sap extracts with dot-ELISA in low dilution exhilbited false positive results,high dilution could eliminate false positive phenomena.Dot-ELISA with rabbitanti-TMV antibody in 1 000 dilution,rabbit-anti-CMV antibody in 500 dilution,tobacco sample sap extracts in 300 dilution and tissue blots were applied for detected TMV and CMV in mosaic tobacco sample collected from field in Qujing,Yunnan.The samples were detected by electrical microscopy in the same time.The results showed that dotELISA and tissue blot-ELISA performed well in detection of TMV and CMV in tobacco sample.The tissue blotELISA is more simple and sensitive than dot-ELISA,and suit for large amount samples routine detection.关键词
点免疫结合法/组织印迹法/TMV/CMVKey words
Dot-immunoblots enzyme-linked immunosorbent assay (Dot-ELISA)/Tissue blot-ELISA分类
农业科技引用本文复制引用
刘勇,杨树军,李天飞..应用点免疫结合法和组织印迹法检测烟草组织中的TMV和CMV[J].烟草科技,2000,(12):42-44,3.基金项目
本研究为云南省“九五”科技攻关项目(95A4-2),云南省烟草公司资助项目的一部分. （95A4-2）
