云南民族大学学报（自然科学版）2017，Vol.26Issue(2)：97-102,6.DOI:12.3969/j.issn.1672-8513.2017.02.001
超声辅助提取桑葚多酚及抗氧化活性分析
Extraction and antioxidant activity of total phenolic compounds from mulberry
摘要
Abstract
The ultrasound-assisted extraction (UAE) of polyphenols from mulberry was optimized by investigating the effects of the ultrasonic time,solid-to-solvent ratio and solvent concentration factors.And their antioxidant activities were evaluated by reducing powers,DPPH free radicals and 2,2'-ABTS free radicals' scavenging assay.The optimal extraction conditions were 70 min for the extraction time,70％ for the ethanol concentration and 1∶30 (g/mL) for the solid-to-solvent radio.The content of polyphenols was 4.014％.The mulberry had antionidant activities in a dose-dependent manner.And after purification by macroporous resins,the antioxidant activities was also tested.The IC50 was 0.730,0.682,0.586 mg/mL and 0.4536,0.556,0.290 mg/mL,respectively.The present study showed that mulberry had strong antioxidant activities and polyphenols were a material basis of mulberry.关键词
桑葚/多酚/超声提取/正交设计方法/抗氧化性Key words
mulberry/polyphenols/ultrasonic assisted extraction/orthogonal design分类
农业科技引用本文复制引用
李柏榆,杨美莲,曹建新,程桂广..超声辅助提取桑葚多酚及抗氧化活性分析[J].云南民族大学学报（自然科学版）,2017,26(2):97-102,6.基金项目
国家自然科学基金(31600274,31460083) （31600274,31460083）
昆明理工大学大学生创新创业项目(201610674100) （201610674100）
昆明理工大学大学生课外创新基金(2015YB064). （2015YB064）
