云南民族大学学报（自然科学版）2017，Vol.26Issue(2)：103-107,5.DOI:12.3969/j.issn.1672-8513.2017.02.002
响应面法优化仙草黄酮提取工艺研究
Optimization for extraction conditions of flavonoids from Mesona Blume with the response surface method
摘要
Abstract
This research aimed to optimize the extraction process of flavonoids from Mesona Blume with the response surface method.On the basis of single-factor experiments,the ethanol concentration,the extraction time and the liquid ratio were selected as the influencing factors,and the box-benhnken designcenter combination method in the experimental design was applied to further optimize the extraction conditions,and the extracting ratio was assayed by the response surface methodology.The sequence of factors influencing the extraction of flavonoids from Mesona Blume from high to low is as follows:ethanol concentration,material liquid and extraction time.The optimal extraction conditions of flavonoids were 60 ％ for the ethanol concentration,5 h for the extraction time and 30 ∶ 1 for the liquid-material ratio.Under the optimal conditions,the maximum extraction rate of flavonoids was 14.37％.The response surface methodology was applied to the optimization of the extraction of flavonoids from Mesona Blume,whose process laid a foundation for the comprehensive utilization of Mesona Blume flavonoids.关键词
仙草/黄酮/提取工艺/响应面法Key words
Mesona Blume/flavonoids/extraction process/response surface methodology分类
生物科学引用本文复制引用
邓婷,黄义松,魏好程,何传波,杨佳洪,熊何健..响应面法优化仙草黄酮提取工艺研究[J].云南民族大学学报（自然科学版）,2017,26(2):103-107,5.基金项目
福建省自然科学基金(2015J01141) （2015J01141）
福建省教育厅科技项目(JA15274). （JA15274）
