中风与神经疾病杂志2017，Vol.34Issue(3)：221-225,5.
一种稳定阿尔茨海默病细胞模型的建立方法
A method for establishing a stable cellular model of Alzheimer's disease
曹方圆 1罗德欣 2黄玉雕2
作者信息
- 1. 哈尔滨医科大学基础医学院形态实验中心,黑龙江 大庆 163319
- 2. 哈尔滨医科大学附属第五医院神经内科,黑龙江 大庆 163319
- 折叠
摘要
Abstract
Objective Aimt to establish a stable model of Alzheimer's disease by using different concentrations of Aβ1-42 to induce hippocampal neurons of the primary culture for hippocampal neurons of SD newborn rats.Methods Extract and culture the primary hippocampal neurons from newborn SD rats within 24 h SD rats;Add to different concentrations of Aβ1-42 to induce the hippocampal neurons and observe cell viability by method of MTT,then choose the ideal hippocampal neurons as Alzheimer's disease cell model;Use the Immunofluorescence(IF)to identify the expression of neuron-specific enolase(NSE)in hippocampal neurons.Results The protein of Aβ1-42 could lead to body deformation,shrinkage and cell membrane integrity damage of hippocampal neuron cell,network structure disappears or fracture between cells,clutter unclear of extracellular matrix and apoptosis of a large number of neurons;The survival rate of hippocampal neurons significantly reduced after induced by the protein of Aβ1-42 and it had a liner relationship between the concentration of Aβ1-42,the cells of SD rats could as a kind of ideal model of Alzheimer's disease cells.The survival rates of experimental groups were(98.55 ± 1.42)%,(90.37 ± 3.25)%,(66.34 ± 2.76)%,(53.23 ± 3.41)% and(41.55 ± 2.37)% by adding different concentrations of Aβ1-42.Conclusion The protein of Aβ1-42 could lead to apoptosis of a large number of neurons;The hippocampal neurons of SD rats can as a kind of ideal model of Alzheimer's disease cells,who the induce concentration of Aβ1-42 is 20 μmol/L.关键词
阿尔茨海默病/Aβ1-42/海马神经元/模型Key words
Alzheimer's disease/Aβ1-42/Hippocampal neuron/Model分类
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曹方圆,罗德欣,黄玉雕..一种稳定阿尔茨海默病细胞模型的建立方法[J].中风与神经疾病杂志,2017,34(3):221-225,5.
