中国动脉硬化杂志2017，Vol.25Issue(3)：230-237,8.
高血压和增龄对大鼠小动脉平滑肌表型转换和miR-143/145表达的影响
The effects of VSMCs phenotype switching and miR-143/145 in arteries during hypertension and aging in rats
摘要
Abstract
Aim This study was designed to explore the characteristics of VSMCs phenotype during aging and hypertension,and how miR-143/145 were influenced.Methods Mesenteric arteries from 1-,3-,9-,and 16-monthold WKY and SHR were isolated,artery histology were evaluated by staining with HE.VSMCs phenotype marker including α-actin,Calponin,and OPN were measured.miR-143/145 were quantified by real-time PCR.Results VSMCs contractile marker α-actin and Calponin expression both reached the peak at 3M and then decreased,while OPN increased with age in SHR with no obvious changes in WKY.The expressions of α-actin,Calponin,and OPN showed significant differences between SHR and the age-matched WKY.miR-143/145 firstly increased with age and then decreased in both WKY and SHR,and miR-143/145 in WKY-3M was significantly higher than the age-matched SHR.Conclusion Aging and hypertension accelerate the VSMCs phenotype switching in arterioles,promoting the synthesis phenotype;miR-143/145 is inversely regulated with contractile VSMCs and could play a role during aging hypertension.关键词
血管平滑肌/表型转换/miRNA/高血压/增龄Key words
Vascular smooth muscle/Phenotype switching/miRNA/Hypertension/Aging分类
医药卫生引用本文复制引用
廖静雯,张琳,张严焱,叶芳,曾凡星,石丽君..高血压和增龄对大鼠小动脉平滑肌表型转换和miR-143/145表达的影响[J].中国动脉硬化杂志,2017,25(3):230-237,8.基金项目
国家体育总局科研课题资助项目(2015B035) （2015B035）
中央高校基本科研业务费专项资金资助项目(2015ZD008) （2015ZD008）
