中国动脉硬化杂志2017，Vol.25Issue(3)：238-243,6.
CXCR4基因过表达HUCB-late EPCs移植对大鼠后肢缺血组织血管新生的影响
Effect of revascularization on human umbilical cordblood-derived endothelial progenitor cells with CXCR4 over-expressed in hind limb ischemic tissue
摘要
Abstract
Aim To evaluate the ability of capillary network formation of HUCB-late EPCs in vitro and in rat hind limb ischemic tissues,after transfected with rAAV-CXCR4 and rAAV-GFP.Methods HUCB-late EPCs were infected with rAAV6-CXCR4、rAAV6-GFP,and the expression of CXCR4 protein was detected by Western Blot analysis.In vitro,evaluate the ability of capillary network formation on Matrigel between the three groups.The mode of mouse hind limb ischemia was set up,by igating the femoral artery and its branches.Following successful establishment,18 rats were divided into three groups randomly:blank group were intramuscularly injected with 500 μL EGM-2 at 6 hours following injury,and control group were intramuscularly injected with 500 μL EGM-2 at 6 hours following injury (contain 1× 106 non-gene transfected HUCB-late EPCs),and experiment group were intramuscularly injected with 500 μL EGM-2 at 6 hours following injury (contain 1 × 106 CXCR4-gene transfected HUCB-late EPCs).After 28 days,capillary network in hind limb muscles and next to muscles were detected by HE stain assay,and CD31 expressed in neovascularization endothelial cells were detected by immune histochemistry assay,and calculate the number of CD31 positive neovascularization,and calculate the number of CD31 positive neovascularization.Results Compared with infected rAAV6-GFP and non-infected group,the expression of CXCR4 protein was up-regulated after infected rAAV6-CXCR4.In vitro,there is no significance of tube area in unit area among three types of HUCB-late EPCs (P＞0.05).In the mode of mouse hind limb ischemia,compared with blank group and control group,CD31 positive cells of new capillary network in experimental group increased obviously(P＜0.05).Conclusion CXCR4 over-expression of HUCB-late EPCs could promote neovascularization in rat hind limb ischemic tissues.关键词
趋化因子受体4/后肢缺血模型/血管新生Key words
Chemokine receptor-4/Hind limb ischemic model/Neovascularization分类
医药卫生引用本文复制引用
张百灵,王照飞,张航,何晋,郑昭芬,彭建强..CXCR4基因过表达HUCB-late EPCs移植对大鼠后肢缺血组织血管新生的影响[J].中国动脉硬化杂志,2017,25(3):238-243,6.基金项目
湖南省卫生厅科研基金课题(C2012-011) （C2012-011）
湖南省教育厅科研项目(15C0828) （15C0828）
