中国动脉硬化杂志2017，Vol.25Issue(3)：244-249,6.
丹参酮ⅡA通过调节自噬小体对ox-LDL诱导内皮细胞氧化应激损伤的保护作用
The protective effect of Tanshinone Ⅱ A on ox-LDL induced endothelial cell oxidative stress injury through regulating autophagosome
摘要
Abstract
Aim Based on the signaling pathway of autophagosome formation to discuss the protective effect and mechanism of Tanshinone Ⅱ A on endothelial cell oxidative stress injury.Methods Culturing EA.hy926 cells in vitro,then randomly dividing these cells into normal group,model group,tanshinone Ⅱ A group,tanshinone Ⅱ A and model group,3-MA group,model and 3-MA group,tanshinone Ⅱ A,model and 3-MA group.Using colorimetric method to test the cells' MDA content and SOD activity of oxidative stress injury.Western blot detecting the expressions of cells'autophagosome formation signaling pathway related protein.Results Compared with normal group,the MDA content is increased (P＜0.01),the SOD activity is decreased (P＜0.01),and the content of LC3-Ⅰ/LC3-Ⅱ protein is increased (P＜0.01) in model group.There is no obvious change of LC3-Ⅰ/LC3-Ⅱ protein content (P＞0.05) in 3-MA group compared with normal group.Compared with model group,the MDA content is decreased (P＜0.01),the SOD activity is increased (P＜0.01) and the content of LC3-Ⅰ/LC3-Ⅱ protein is increased (P＜0.01) in tanshinone Ⅱ A and model group.The MDA content is increased (P＜0.01),the SOD activity is decreased (P＜0.01),and the content of LC3-Ⅰ/LC3-Ⅱ protein is decreased (P＜0.01) in model and 3-MA group compared with model group.Compared with tanshinone ⅡA and model group,the MDA content is increased (P＜0.01),the SOD activity is decreased (P＜0.01),and the content of LC3-Ⅰ/LC3-Ⅱ protein is decreased (P＜0.01) in tanshinone Ⅱ A,model and 3-MA group.Compared with normal group,the expression of Atg3,Atg4b,Atg7 are increased significantly in model group (P＜0.05 or P＜0.01).Compared with model group,the expression of Atg3,Atg7,Atg5-Atg12 are increased significantly in tanshinone Ⅱ A and model group (P＜0.05 or P＜0.01).Compared with tanshinone Ⅱ A and model group,the expression of Atg3,Atg7,Atg5Atg12 are decreased significantly in tanshinone Ⅱ A,model and 3-MA group (P＜0.05 or P＜0.01).Conclusion Tanshinone Ⅱ A may regulate the proteins of signaling pathway of autophagosome formation as well as the Atgl2-Atg5 pathway and LC3-PE pathway of EA.hy926 cell to play its protective biological activities for EA.hy926 cells against oxidative stress damage,then prevent and treat the development of As.关键词
丹参酮ⅡA/内皮细胞/动脉粥样硬化/氧化应激/自噬小体Key words
Tanshinone Ⅱ A/Endothelial cell/Atherosclerosis/Oxidative stress/Autophagosome formation分类
医药卫生引用本文复制引用
张妮,曹慧敏,宋囡,杨关林,贾连群,张哲..丹参酮ⅡA通过调节自噬小体对ox-LDL诱导内皮细胞氧化应激损伤的保护作用[J].中国动脉硬化杂志,2017,25(3):244-249,6.基金项目
辽宁省自然科学基金项目(2015020394) （2015020394）
辽宁省高等学校优秀人才支持计划(LR2015041) （LR2015041）
辽宁中医药大学2015年度大学生创新创业训练计划项目(201510162000066) （201510162000066）
