中国动脉硬化杂志2017，Vol.25Issue(3)：254-258,5.
同型半胱氨酸对巨噬细胞CD147表达的影响及瑞舒伐他汀的干预作用
Effects of homocysteine on CD147 expression in macrophages and the intervention effect of rosuvastatin
殷永军 1杜大勇 2李运田 1柳杨 2宗家欣 2李巍 1张雁斌2
作者信息
- 1. 南方医科大学,广东省广州市510515
- 2. 南方医科大学附属解放军第305医院心脏中心,北京市100017
- 折叠
摘要
Abstract
Aim To investigate the influence of homocysteine (Hcy) on CD147 expression in human U-937 macrophages and intervention effect of rosuvastatin.Methods Human U-937 macrophages induced by PMA were treated with 0,50,100 and 500 μmol/L Hcy for 48 hours respectively,CD147 expression was then analyzed using Western blot and RT-PCR.Different concentrations of rosuvastatin (10-s～ 10-5 mol/L) were added when U-937 macrophages were treated with 500 μmol/L Hcy.CD147 expression was then examined after incubating for 48 h.Cellular immumofluorescence method was used to detect the expression of CD147.Results Hcy significantly increased the expression of CD147 in a dose-dependent manner.Rosuvastatin (10-7～ 10-5 mol/L) significantly reversed the expression of Hcy-induced CD147 in U-937 macrophages in a dose-dependent manner.Cellular immumofluorescence demonstrated that the CD147 expression of U-937 macrophages was significantly increased by Hcy compared with control group,rosuvastatin (10-5 mol/L) cloud reverse the effect.Conclusion Hcy (100～500 μmol/L) significantly increased the production of CD147 in PMA-induced U-937 macrophages in a dose-dependent manner.Additional extracellular rosuvastatin can decrease the expression of CD147 in PMA-induced U-937 macrophages induced by Hcy.关键词
同型半胱氨酸/巨噬细胞/CD147/瑞舒伐他汀Key words
Homocysteine/Macrophages/CD147/Rosuvastatin分类
医药卫生引用本文复制引用
殷永军,杜大勇,李运田,柳杨,宗家欣,李巍,张雁斌..同型半胱氨酸对巨噬细胞CD147表达的影响及瑞舒伐他汀的干预作用[J].中国动脉硬化杂志,2017,25(3):254-258,5.
