局解手术学杂志2017，Vol.26Issue(2)：79-82,4.DOI:10.11659/jjssx.06E016020
大鼠肝部分切除术后腹腔神经丛阻滞抑制早期应激反应
Postoperative early stress response alleviated by celiac plexus block after partial hepatectomy in rats
摘要
Abstract
Objective To investigate the effects of neurolytic celiac plexus block(NCPB) on stress response during the early stage of partial hepatectomy(PH) postoperation in rats.Methods Thirty healthy SPF-grade male Sprague-Dawley rats were randomly divided into control group and NCPB group,and then were constructed the 70％ PH model.NCPB group received NCPB with 0.5 ％ lidocaine before closing abdominal,and control group received the same procedures,but 0.9％ physiological saline was injected instead of 0.5 ％ lidocaine.The serum concentration of CRP,GC,ACTH,NA and AD,and the levels of serum TNF-α and IL-1β were determined at 6 hours,12 hours and 24 hours after PH.The western blot assay was performed to examine the expression of GR in the peripheral blood mononuclear cells (PBMCs).Results The obvious stress response was produced within 24 hours after PH in rats,postoperative serum CRP,GC,ACTH,NA and AD concentrations increased in the different extent,but the NCPB group were significantly lower than those in the control group in the corresponding time point (P ＜0.01 or P ＜0.05).The expression levels of GR in PBMCs in the control group were significantly decreased after PH,and markedly lower than those in the NCPB group in the corresponding time point(P ＜ 0.01).The serum TNF-o and IL-1β levels were gradually increased in the control group and NCPB group within 24 hours after PH.But at the respective time points,the levels in the NCPB group were significantly lower than those in control group(P ＜ 0.01 or P ＜ 0.05).Conclusion Intraoperation NCPB can effectively inhibit postoperative stress response at the early stage after PH,and thereby help to reduce the degree of systemic inflammatory response.关键词
肝部分切除术/腹腔神经丛阻滞/应激反应Key words
partial hepatectomy/neurolytic celiac plexus block/stress response分类
医药卫生引用本文复制引用
魏晓红,林露,李军,牛洁,刘小燕..大鼠肝部分切除术后腹腔神经丛阻滞抑制早期应激反应[J].局解手术学杂志,2017,26(2):79-82,4.基金项目
成都军区医学科学技术研究计划重点项目(B14010) （B14010）
成都军区总医院院管课题(2013YG-B010) （2013YG-B010）
