局解手术学杂志2017，Vol.26Issue(2)：89-93,5.DOI:10.11659/jjssx.10E016046
白术内酯Ⅰ抑制卵巢癌SK-OV-3与OVCAR-3细胞增殖作用机制的研究
Mechanisms and proliferation inhibitory effects of atractylenolide Ⅰ on SK-OV-3 and OVCAR-3 ovarian cancer cell
龙方懿 1贾萍 1王华飞 1卿轶 1何梦婕 1王晓丽1
作者信息
- 1. 四川省妇幼保健院药学部,四川成都610041
- 折叠
摘要
Abstract
Objective To investigate the mechanisms and proliferation inhibitory effects of atractylenolide Ⅰ on SK-OV-3 and OVCAR-3 ovarian cancer cell.Methods SK-OV-3 and OVCAR-3 cells were treated with atractylenolide I with various concentrations at 24 hours,48 hours and 72 hours,and the changes in proliferation were detected by MTT assay.The cell cycles were measured by PI staining and flow cytometry,and the expressions of cyclin D1 and CDK1 were detected by ELISA assay.Western blot was then applied to investigate the effects of atractylenolide Ⅰ on PI3K/AKT signaling pathway in SK-OV-3 and OVCAR-3 cells.Results Atractylenolide I could significantly inhibit the proliferation of SK-OV-3 and OVCAR-3 cells,and its inhibitory effects were concentration and time dependent.In addition,atractylenolide I could also significantly reduce the proportion of cells in S phase and increase the proportion of cells in G2/M phase,and these effects were associated with the down-regulation of CDK1.The results of Western blot indicated that PI3K/AKT signaling pathway was involved in the inhibitory effects of atractylenolide Ⅰ on proliferation and cell cycle.Conclusion Atractylenolide I can down-regulate the expression of CDK1 in ovarian cancer SK-OV-3 and OVCAR-3 cells through PI3K/AKT pathway,which led to cell cycle arrest in G2/M phase,and played an important role in proliferation inhibition of tumor cells.关键词
白术内酯Ⅰ/细胞增殖/细胞周期/PI3K/AKT/卵巢癌Key words
atractylenolide Ⅰ/proliferation/cell cycle/PI3K/AKT/ovarian cancer分类
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龙方懿,贾萍,王华飞,卿轶,何梦婕,王晓丽..白术内酯Ⅰ抑制卵巢癌SK-OV-3与OVCAR-3细胞增殖作用机制的研究[J].局解手术学杂志,2017,26(2):89-93,5.
