中国临床药理学杂志2017，Vol.33Issue(6)：518-521,4.DOI:10.13699/j.cnki.1001-6821.2017.06.011
心脑舒通胶囊对蒙古沙鼠全脑缺血再灌注损伤模型的神经保护作用
Neuroprotective effects of Xinnaoshutong capsule on the brain ischemia-reperfusion injury in Mongolian gerbils
摘要
Abstract
Objective To investigate the protective effect and the possible mechanism of Xinnaoshutong capsule on brain damage in Mongolian gerbil exposed to brain ischemia.Methods Mongolian gerbils were divided into 4 groups randomly:sham operation group,model group,experimental-H and experimental-L groups (Xinnaoshutong 56,14 mg · kg-1).Gerbil ischemia reperfusion(I/R) model was established by bilateral common carotid artery occlusion for 30 min followed by reperfusion for 6 days.Daily intragastric administration for 1 times,last for 7 days.The structure of neurons in hippocampal CA1 region was evaluated by Hematoxylin-Eosin (HE) staining.The expression of glial fibrillary acidic protein (GFAP) in the hippocampus CA1 area was evaluated by immunohistochemical staining.Results Compared with the sham operation group of survival rate(100％),the gerbil survival rate of the 66.7％ in model group significantly decreased (P ＜ 0.01).Compared with the sham operation group on nerve cell proportion of (100.00 ± 16.72) ％,nerve cell proportion was (45.56 ± 16.72) ％ in model group with significantly decreased (P ＜ 0.01).The hippocampus CA1 area neurons in the brain to arrange sparse disorder in model group.The expression of GFAP increased obviously in model group.Compared with the model group on the survival rate of cerebral ischemia gerbils of 66.7 ％,can make the survival rate of cerebral ischemia gerbils in Xinnaoshutong group 86.7％ increased significantly (P ＜0.01).And Xinnaoshutong can improve the hippocampus CA1 area neurons in the brain tissue form.Compared with the model group on the proportion of nerve cells of (45.56 ±16.72)％,the proportion of nerve cells in experimental-H group (79.84 ± 15.39)％ significantly increased(P ＜ 0.01).Xinnaoshutong can inhibit the expression of GFAP.Conclusion Xinnaoshutong has positively protective effect on brain ischemia-reperfusion injury,it may be relevant with inhibiting astrocyte activation and anti-apoptosis,which may be one of the mechanism of treatment of stroke.关键词
脑缺血再灌/心脑舒通胶囊/神经细胞/星形胶质细胞活化/蒙古沙鼠Key words
brain ischemia reperfusion/Xinnaoshutong capsule/neurocyte/astrocyte activation/Mongolian gerbils分类
医药卫生引用本文复制引用
王金鑫,卢国彦,郭虹,柴丽娟,张玥,王少峡,胡利民..心脑舒通胶囊对蒙古沙鼠全脑缺血再灌注损伤模型的神经保护作用[J].中国临床药理学杂志,2017,33(6):518-521,4.基金项目
科技部国家重大新药创制基金资助项目(2011ZX09201201-033) （2011ZX09201201-033）
国家自然科学基金资助项目(8157140605) （8157140605）
