中国临床药理学杂志2017，Vol.33Issue(6)：522-525,534,5.DOI:10.13699/j.cnki.1001-6821.2017.06.012
注射用血栓通及其有效组分对缺氧/复氧损伤神经元保护作用
Protective effects of Xueshuantong injection and its effective constituents on neurons with hypoxia/reoxygenation injury
摘要
Abstract
Objective To compare the protective effects of Xueshuantong injection and its effective constituents on neurons cells with hypoxia/ reoxygenation(HL/R) injury.Methods Neuro-2a cells were cultured and divided into control group,model group,administration group (Panaxadiolsaponin group,Panaxatriolsaponin group and Xueshuantong group) and compatibility group.The model of H/R was established.The H/R cells were treated with different concentrations of Panaxadiolsaponins,Panaxtrolsaponins and Xueshuantong (50,100,200,400 and 800 mg · L-1) for 24 h.The cells in the compatibility group of Panaxadiolsaponins,and Panaxtrolsaponins(100 mg · L-1) were treated for 24 h.The cell viability and the leakage of lactate dehydrogenase (LDH) in each group were detected by CCK-8 and the leakage of lactate dehydrogenase (LDH) Kit method.Results Compared to model group on the survival rate of cells with (51.13 ± 1.74) ％,the survival rates of cells with (24.27 ±4.88)％,(1.13 ± 0.3)％,(0.93 ±0.30)％ in Panaxadiolsaponin group at the concentrations of 200,400,800 mg · L-1 were decreased(P ＜0.01).Compared to model group on the leakage rate of LDH with (260.97 ± 10.86)％,the leakage rates of LDH with (319.33 ± 31.37)％,(599.32 ± 34.11)％,(879.58 ±47.20)％ in Panaxadiolsaponin group at the three concentrations were increased(P ＜0.01).Compared to model group on the survival rate of cells with (51.16 ± 6.32)％ and the leakage rate of LDH with (361.20 ± 26.43) ％,the survival rates of cells with (66.26 ± 3.15) ％,(60.23 ± 1.93) ％,(62.22 ± 4.79) ％ were increased and the leakage rates of LDH with (295.97 ± 23.62) ％,(235.97 ± 43.80) ％,(173.33 ± 26.53) ％ were decreased in Panaxtrolsaponin group at the three concentrations (P ＜ 0.01).Compared to model group on the survival rate of cells with (50.01 ±2.83)％ and the leakage rate of LDH with(270 ±74.24)％,the survival rates of cells with (58.63 ± 6.07) ％,(65.25 ± 8.80) ％,(66.38 ± 8.62) ％ were increased and the leakage rates of LDH with (171.45 ± 13.69) ％,(190.55 ± 17.90) ％,(187.07 ± 30.09) ％ were decreased in Xueshuantong group at the concentrations of 100,200,400 mg · L-1 (P ＜ 0.01).Compared to model group on the survival rate of cells with (37.29 ± 3.73) ％,the survival rates of cells with (46.57 ± 1.27) ％,(43.29 ± 2.29) ％ in the second and fifth compatibility group were increased and the cell survival rate with (27.92 ± 2.89)％ was decreased in the fourth compatibility group significantly (P ＜ 0.05,P ＜ 0.01).Compared to model group on the leakage rate of LDH with (252.67 ± 9.35) ％,the rates of LDH leakage with (211.17 ± 7.73) ％,(227.17 ± 13.91) ％ in the second and fifth compatibility group were decreased and the rate of LDH leakage with (295.83 ± 12.77) ％ was significantly increased in the fourth compatibility group (P ＜ 0.05,P ＜ 0.01).Conclusion Xueshuantong injection and its effective components had a protective effect on neurons cells with H/R injury.关键词
注射用血栓通/人参二醇皂苷/人参三醇皂苷/神经保护Key words
Xueshuantong injection/Panaxadiolsaponin/Panaxtrolsaponin/neuroprotection分类
医药卫生引用本文复制引用
徐杨杨,郭虹,赵莼,王少峡,柴丽娟,袁庆,王金鑫,王富江,胡利民..注射用血栓通及其有效组分对缺氧/复氧损伤神经元保护作用[J].中国临床药理学杂志,2017,33(6):522-525,534,5.基金项目
国家自然科学基金资助项目(81573644) （81573644）
国家重大新药创制注射用血栓通技术升级研究基金资助项目(2012ZX09101201) （2012ZX09101201）
天津市应用基础与前沿技术研究计划基金资助项目(14JCYBJC28900) （14JCYBJC28900）
广西科学研究与技术开发计划基金资助项目(14125008-2-5) （14125008-2-5）
