中国临床药理学杂志2017，Vol.33Issue(6)：535-538,4.DOI:10.13699/j.cnki.1001-6821.2017.06.015
肾复舒颗粒对顺铂肾毒性小鼠的保护作用研究
Protective effect of Shenfushu granules on nephrotoxicity induced by cisplatin in mice
摘要
Abstract
Objective To evaluate the impact of Shenfushu granules on cisplatin-induced renal dysfunction and explore the possible mechanisms.Methods Male institute of cancer research(ICR) mice were randomly divided into 4 groups:control group (0.9％ NaC1),Shenfushu group (Shenfushu granules 1 g · kg-1),cisplatin group(0.9％ NaC1 for 9 d and a single dose of cisplatin 20 mg · kg-1 was given by intraperitoneal injection on 7th day),Shenfushu + cisplatin group (Shenfushu granules 1 g · kg-1 gavage for 9 d and a single dose of cisplatin 20 mg · kg-1 was given by intraperitoneal injection on 7th day).Animals were euthanized 72 h following cisplatin dosing.Biochemical parametem including serum creatinine and blood urea nitrogen levels were analyzed by automatic biochemical analyzer.Hematoxylin-eosin (HE) staining was performed inhistopathological examination.The peroxidative product malondialdehyde (MDA) and antioxidant enzymes glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) were detected according to kits.Protein expressions of nuclear factor-E2-related factor 2 (Nrf2),hemeoxygenase 1 (HO-1),quinine oxidoreductase 1 (NQO1),multidrug resistance protein 2 (MRP2) and multidrug and toxic compound extrusion protein 1 (MATE1) were measured in western blot analysis.Platinum contents in kidney were determined with inductively coupled plasma mass spectrometry (ICP-MS) method.Results The serum creatinine in cisplatin group and Shenfushu + cisplatin group were (12.14 ± 4.64),(7.60 ± 3.49) μmol · L-1;blood urea nitrogen were (34.12 ±9.48),(15.92 ±4.33) mmol · L-1;GSH-Px were (78.26 ±7.02),(95.87 ± 14.08) U· mg-1 protein;SOD were (8.85 ±3.99),(12.68 ±3.62) U · mg-1 protein,MDA were (6.96 ±2.25),(4.33 ± 1.50) nmol · mg-1 protein;platinum contents were (12.56 ± 1.30),(9.67 ± 0.97) ng · mg-1 tissue.Western blotting analysis revealed that Shenfushu granules enhanced expressions of Nrf2-mediated antioxidant pathway and efflux transporter in cisplatin-exposed animals.Conclusion Shenfushu granules exhibited renal protective effects on cisplatin-induced renal impairment,possibly associated with its modulating on Nrf2-mediated antioxidant pathway and efflux transporters.关键词
顺铂/肾毒性/核因子E2相关因子2/转运体:肾复舒颗粒Key words
cisplatin/nephrotoxicity/nuclear factor-E2-related factor 2/transporters/Shenfushu granules分类
医药卫生引用本文复制引用
曹思思,龚双,颜苗,蒋云生,樊新荣,张毕奎..肾复舒颗粒对顺铂肾毒性小鼠的保护作用研究[J].中国临床药理学杂志,2017,33(6):535-538,4.基金项目
国家自然科学基金资助项目(81202985 （）
81573686 （）
81373708) （）
