中国免疫学杂志2017，Vol.33Issue(4)：494-497,4.DOI:10.3969/j.issn.1000-484X.2017.04.003
IFN-α通过IFI16抑制人脑血管外膜成纤维细胞增殖并促进细胞凋亡
Interferon alpha suppresses proliferation and induces apoptosis of human brain vascular adventitial fibroblasts via IFI16
摘要
Abstract
Objective:To study interferon alpha (IFN-α) inhibition of proliferation and apoptosis induction of human brain vascular adventitial fibroblasts(HBVAFs)via IFI16.Methods:Cultured HBVAFs were treated with transfection IFI16 siRNA and/or IFN-α in vitro instantaneously.The protein and mRNA levels of IFI16,P53,P21 were measured by Western blot and Real-time PCR.MTT was used to detect the cell proliferation of the HBVAFs.Cell cycle and apoptosis were analyzed by flow cytometry.Results:IFN-α with terminal concentration of 2 000-5 000 kU/L induce significantly expression of IFI16 in HBVAFs,without any significant difference.Stimulated with 2 000 kU/L IFN-α up-regulated the expression of P53,P21 at protein and mRNA levels,and inhibited the cell proliferation and promote cells apoptosis in HBVAFs.Such effect was restrained by transfection with IFI16 siRNA into HBVAFs.Conclusion:IFN-α inhibits HBVAFs proliferation and induces apoptosis may partly relate to the increased IFI16 expression.关键词
干扰素诱导蛋白/干扰素-α/血管外膜成纤维细胞/增殖/凋亡Key words
Interferon-inducible protein/IFN-α/Vascular adventitial fibroblasts/Proliferation/Apoptosis分类
医药卫生引用本文复制引用
黄晶,宋方,龙向淑,田茂波,吴强..IFN-α通过IFI16抑制人脑血管外膜成纤维细胞增殖并促进细胞凋亡[J].中国免疫学杂志,2017,33(4):494-497,4.基金项目
本文为国家自然科学基金项目(No.81260030). （No.81260030）
