中国免疫学杂志2017，Vol.33Issue(4)：533-536,541,5.DOI:10.3969/j.issn.1000-484X.2017.04.011
基于PI3K/AKT/mTOR通路、HIF-1α、ES观察新风胶囊对佐剂关节炎大鼠滑膜血管新生的影响
Effects of Xinfeng Capsule on synovial angiogenesis based PI3K/AKT/mTOR pathway,HIF-1α,ES in rats model of adjuvant arthritis
摘要
Abstract
Objective:To observe the effect of Xinfeng Capsule (XFC) on synovial membrane PI3K/AKT/mTOR pathway in adjuvant arthritis (AA) rats.Methods:Rats were randomly divided into normal control group,model control group,methotrexate group,tripterygium glycoside group and XFC group.The expressions of IL-6,IL-10,HIF-1α and VEGF-A were detected by immunohistochemical method,and the expressions of PI3K,AKT1 and p-AKT1 were detected by immunoblotting.Results:Expression of AKT1,mTOR and ES proteins in synovial blood vessels.The expression of IL-6,VEGF,HIF-1α and synovial membrane PI3K,AKT1,p-AKT1,mTOR,ES in the model group were significantly higher than those in the control group The expression of PI3K,p-AKT1,mTOR,ES in synovial membrane decreased,and IL-10 in serum increased in XFC group,and the expression of HIF-1α,IL-6 and VEGF-A were decreased.Conclusion:XFC can improve angiogenesis in AA synovium by regulating PI3K/AKT/mTOR signaling pathway,HIF-1α and ES expression.关键词
佐剂关节炎/血管新生/PI3K/AKT/mTOR通路/新风胶囊Key words
Adjuvant arthritis/Angiogenesis/PI3K/AKT/mTOR pathway/Xinfeng Capsule分类
医药卫生引用本文复制引用
张晓军,刘健,万磊,黄传兵..基于PI3K/AKT/mTOR通路、HIF-1α、ES观察新风胶囊对佐剂关节炎大鼠滑膜血管新生的影响[J].中国免疫学杂志,2017,33(4):533-536,541,5.基金项目
本文为安徽省科技厅项目(1604f0804030)、国家自然科学基金青年项目(81403388)、安徽省自然基金项目(1508085QH159)、安徽省重点实验室:现代中医内科应用基础与开发研究(1606c08238)和安徽中医药大学校级基金(2014qn025)资助项目. （1604f0804030）
