中国免疫学杂志2017，Vol.33Issue(4)：542-544,3.DOI:10.3969/j.issn.1000-484X.2017.04.013
扶正解毒通络方对人肝癌HepG2细胞MMP-2、MMP-9表达及细胞侵袭作用的影响
Effect of Fuzheng Jiedu Tongluofang on MMP-2 and MMP-9 and invasion of human hepatoma HepG2 cells
摘要
Abstract
Objective:To investigate the effect and mechanism of Fuzheng Jiedu Tongluofang on the expression of MMP-2,MMP-9 and invasion of human hepatoma HepG2 cell.Methods:Hepatioma HepG2 cell was treated with Fuzheng Jiedu Tongluofang.The cell viability was measured by CCK8.The invasion of HepG2 was detected by Transwell assay.The expression of MMP-2 and MMP-9 was analyzed by ELISA.Results:In the CCK8 assay,Fuzheng Jiedu Tongluofang could inhibit the proliferation of HepG2 cells at a density and time measure.In the Transwell assay,the inhibitory rate of invasion was 52.45% when treated with Fuzheng Jiedu Tongluofang at a concentration of 2.65 mg/ml. The ELISA assay indicated that the expression of MMP-2 and MMP-9 decreased significantly after treated with Fuzheng Jiedu Tongluofang(P<0.05). Conclusion:Fuzheng Jiedu Tongluofang suppressed the invasion of HepG2 with the possible mechanism of down-regulating the expression of MMP-2 and MMP-9 in HepG2.关键词
扶正解毒通络方/肝癌HepG2细胞/MMP-2/MMP-9/细胞侵袭Key words
Fuzheng Jiedu Tongluofang/Hepatioma HepG2 cells/MMP-2/MMP-9/Invasion分类
医药卫生引用本文复制引用
张景洲,冯相伟,孟繁平..扶正解毒通络方对人肝癌HepG2细胞MMP-2、MMP-9表达及细胞侵袭作用的影响[J].中国免疫学杂志,2017,33(4):542-544,3.基金项目
本文受吉林省科技发展规划项目(330201Q00)资助. （330201Q00）
