农学学报2017，Vol.7Issue(1)：32-38,7.
草莓采后灰霉病生防菌Burkholderia contaminans培养基优化分析
Medium Optimization Analysis of Biocontrol Bacteria Burkholderia contaminans B-1 Against Fruit Postharvest Grey Mould
摘要
Abstract
This objective was to discover the culture medium formulation of Burkholderia contaminans with the biggest biomass yield under shake flask conditions.In this experiment,we used the multi-factor quadratic general rotary unitized design method to study the effects of varied carbon sources,nitrogen sources and inorganic salt types & concentrations on strain growth,and finally determined the proper formulation.The optimal medium formulation was as follows:malt powder 2.11％,yeast extract 1.90％,KH2PO4 0.05％ and MnSO4 0.05％.It had been proved that the biomass of B.contaminans reached the maximum under this condition and showed better optimization effects by further detection.This optimization analysis will pave the way for establishing high yield fermentation process of B.contaminans,and facilitate its application in afterharvest biocontrol of fruits and vegetables,and industrialized development as well.关键词
Burkholderia contaminans B-1/培养基/二次通用旋转组合/优化分析Key words
Burkholderia contaminans B-1/Culture Medium/Quadratic General Rotary Combination/Optimization Analysis分类
农业科技引用本文复制引用
施俊凤,孙常青,李静..草莓采后灰霉病生防菌Burkholderia contaminans培养基优化分析[J].农学学报,2017,7(1):32-38,7.基金项目
山西省科技攻关项目“拮抗菌Burkholderia contaminans对草莓采后生防技术的研究”(20140311025-2) （20140311025-2）
山西省农业科学院博士基金“拮抗菌Burkholderia contaminans对草莓采后生防潜力研究”(YBSJJ1414) （YBSJJ1414）
山西省农业科学院博士后基金“生防菌株对果实采后灰霉病防治机理研究”(BSH-2015JJ-003). （BSH-2015JJ-003）
