中国水产科学2017，Vol.24Issue(1)：11-21,11.DOI:10.3724/SP.J.1118.2017.16163
牙鲆三个同源纯系的生长和遗传性状比较
Growth and genetic analysis among three homologous inbred strains of Paralichthys olivaceus
摘要
Abstract
Establishing meiotic gynogenesis,mitotic gynogenesis,and inbred lines is important for trait purification during selective fish breeding.Yet how these three genetic pathways affect heredity remains the focus of much genetics research.In this study,we used the female parents from Paralichthys olivaceus family F09119 to establish mitotic gynogenesis (F1346),meiotic gynogenesis (F1324),and an inbred line (F1313) and analyzed their growth and genetic characteristics.Body length,body width,and weight were measured in the three families from 85 to 388 d post fertilization (dpf).Results on 338 dpf showed that growth rate of F1346 was the highest [body length,(28.89±2.77) cm;body width,(10.00±1.21) cm;and weight,(254.91483.11) g] and that ofF1324 was the lowest (P＜0.05).Survival rate ofF1324 on 263 dpf was the highest (46.50％) and that ofF1346 was the lowest (26.80％).The numbers of alleles for mitotic gynogenesis,meiotic gynogenesis,and the inbred line were 2.0,2.0 and 2.35,respectively.The effective numbers of alleles were 1.87,1.95,and 2.1;polymorphic information content values were 0.35,0.36,and 0.4;and mean observed heterozygosity values were 0,0.8155,and 0.6366,respectively,indicating that genetic variation of mitotic gynogenesis and meiotic gynogenesis was less than that of the inbred line.The trend for unbiased expected homozygosity was F1313 (0.503)＜F1324 (0.5105)＜F1346 (0.532) and that of unbiased expected heterozygosity was F 1313 (0.4967)＞F1324 (0.4894)＞F 1346 (0.4679).Homozygosity of meiotic gynogenesis was 1.0421-fold higher than that of meiotic gynogenesis and 1.0577-fold higher than that of the inbred line,whereas that of meiotic gynogenesis was 1.0149-fold higher than that of the inbred line.The genetic distance between F1346 and F1313 was longest (0.1568) but genetic similarity was the lowest (0.8549),whereas the genetic distance between F1346 and F1324 was the shortest (0.0406),and genetic similarity was the greatest (0.9602).These results provide a theoretical basis for establishing an inbred line of P.olivaceus and analyses of genetic variations among meiotic gynogenesis,mitotic gynogenesis,and the inbred line.关键词
牙鲆/卵裂雌核发育系/减数雌核发育系/近交系/表型性状/遗传性状Key words
Paralichthys olivaceus/meiotic gynogenesis line/mitotic gynogenesis line/inbred line/phenotypic character/genetic character分类
农业科技引用本文复制引用
田永胜,段会敏,李祥孔,李洪,宋莉妮,陈红林,陈张帆,唐江,陈松林..牙鲆三个同源纯系的生长和遗传性状比较[J].中国水产科学,2017,24(1):11-21,11.基金项目
国家863高技术研究发展计划项目(2012AA10A408) （2012AA10A408）
青岛海洋科学与技术国家实验室鳌山科技创新计划项目(2015ASKJ02) （2015ASKJ02）
青岛市南区科技发展资金项目(2015-6-023-ZH). （2015-6-023-ZH）
