中国水产科学2017，Vol.24Issue(1)：22-30,9.DOI:10.3724/SP.J.1118.2017.16071
半滑舌鳎抗哈维氏弧菌病相关微卫星标记筛选及QTL定位
Detection of SSR and a QTL analysis of Vibrio harveyi resistance in Cynoglossus semilaevis
摘要
Abstract
The bacterium Vibrio harveyi has caused tremendous losses in the Cynoglossus semilaevis aquaculture industry.The fundamental approach has been to cultivate a new disease-resistant strain by combining traditional breeding methods with molecular techniques.In this study,bulked segregant analysis and quantitative trait loci (QTL) mapping were used to screen for disease-resistance markers.A total of 100 individuals were selected to form the F1412 family (nomenclature rule:F + year + family number:survival rate,52.22％),which was challenged with V.harveyi,and 169 microsatellite loci were detected across all chromosomes.Following the genomic scan,the scaffold479_23523 marker in the DNA pool was significantly different between the resistant and susceptible groups (P=0.000006).Ninety-four individuals were genotyped using all 32 simple sequence repeat markers on LG18,where scaffold479_23523 was located.Three new linkage groups (LG18,LG18F,and LG18M) were identified.Furthermore,two different analytical models were applied to perform a single marker analysis and composite interval mapping with different levels of significance in LG18,LG18F,and LG18M,respectively.In model 1,three significant markers (scaffold4475_71287,scx9-1,and cyse80) and one very significant marker (scaffold080437) were identified,and the qE-F1 resistance-related QTL was detected.The scaffold479_23523 marker was the left LG18F marker with a p-value of 0.0516 in model 1.Model 2 detected four significant markers (hncysell0,scaffold414_19940,scaffold4475_71287,and cyse80),one very significant marker (scaffold08043),and the qE-M1 and qE-M2 QTLs.Both scaffold080437 markers were significantly different (P＜0.001) in the two models.Four markers (scaffold080437,scaffold479_23523,scaffold4475_71287,and cyse80) may be closely associated with resistance to V.harveyi infection in C.semilaevis.qE-F1 explained 87.36％ of the phenotypic variance and contained G18M qE-M1 and qE-M2.Thus,qE-F1 was considered a major candidate region for V.harveyi resistance.After scanning the C.semilaevis genome,three immunity-related genes,such as meteorin-like,the WD repeat domain phosphoinositide interacting 2,and Toni beta-propeller repeat containing 1,were detected inside qE-F1.This is the first study to identify V.harveyi resistance-related markers and conduct a related QTL analysis in C.semilaevis.These results provide a foundation for selective breeding of disease-resistant C.semilaevis.关键词
半滑舌鳎/哈维氏弧菌/微卫星标记SSR/混合分离子分析法BSA/数量性状基因座QTLKey words
Cynoglossus semilaevis/Vibrio harveyi/simple sequence repeats (SSR)/bulked segregant analysis (BSA)/quantitative trait loci (QTL)分类
农业科技引用本文复制引用
戴欢,刘洋,王文文,位战飞,高进,高峰涛,李祥孔,陈松林..半滑舌鳎抗哈维氏弧菌病相关微卫星标记筛选及QTL定位[J].中国水产科学,2017,24(1):22-30,9.基金项目
国家自然科学基金项目(31530078) （31530078）
山东省农业良种工程重大课题和山东省泰山学者攀登计划专项. （）
