中国水产科学2017，Vol.24Issue(1)：57-64,8.DOI:10.3724/SP.J.1118.2017.16146
盐度和胁迫时间对九孔鲍α-淀粉酶基因表达量的联合效应分析
Synergistic effects of salinity and stress duration on alpha-amylase expression in Haliotis diversicolor supertexta
摘要
Abstract
Haliotis diversicolor supertexta is mainly distributed in coastal waters off southern China and is a warmwater,economically important shellfish.H.diversicolor supertexta is characterized by rapid growth,a short breeding cycle,suitability for high density cultivation,and is nutritious,so there is high demand among consumers.Guangdong is often affected by typhoons,so more rain falls there than in other provinces.Sea salinity drops when rainfall increases,and the osmotic pressure in aquatic animals varies because of salinity changes,which can result in massive mortalities of abalone.H.diversicolor supertexta is a local species in Guangdong,making it particularly susceptible.Response surface methodology (RSM) is a collection of statistical and mathematical techniques to optimize a process.This methodology generates an empirical mathematical model that defines the effect of an independent variable on a response of interest to optimize the response.RSM has been applied in ecotoxicological studies of marine bivalve larvae and to investigate the effects of environmental variables on embryonic and larval development.RSM has not been used in any gastropod nutritional studies.In this study,the synergistic effects of salinity and stress duration on alpha-amylase gene expression were assessed using central composite design and RSM.The results show that the linear effects of salinity on alpha-amylase gene expression were not significant (P＞0.05),but the linear effect of stress duration was significant (P＜0.05),suggesting that expression of the amylase gene was affected by stress duration.In addition,the quadratic effects of salinity on amylase gene expression were significant (P＜0.05),but the quadratic effects of stress duration on amylase gene expression were not significant (P＞0.05).No synergism was detected between salinity and stress duration.The RSM model equation for the relationship between amylase gene expression and the two factors was established (R2=80.05％).The Adj.R2 value was 65.80％,and the Pre.R2 values was 50.18％,suggesting a good model fit for prediction.Maximal amylase gene expression was observed when salinity was 31,and stress duration was 36 h.H.diversicolor supertexta is affected by many factors,as it is cultured in a marine environment.Additional factors should be studied in the future.关键词
九孔鲍/α-蛋白酶/盐度/胁迫时问/基因表达量/联合效应Key words
Haliotis diversicolor supertexta/alpha-amylase/salinity/stress duration/gene expression/synergistic effects分类
农业科技引用本文复制引用
钱佳慧,栗志民,刘建勇,艾加林..盐度和胁迫时间对九孔鲍α-淀粉酶基因表达量的联合效应分析[J].中国水产科学,2017,24(1):57-64,8.基金项目
广东省省级科技计划项目(2015A020209196) （2015A020209196）
广东省海洋渔业科技与产业发展专项科技攻关与研发项目(A201408A04) （A201408A04）
深圳市未来产业项目(2015A020209196). （2015A020209196）
