中国水产科学2017，Vol.24Issue(1)：65-72,8.DOI:10.3724/SP.J.1118.2017.16110
急性低盐胁迫下红鳍东方纯幼鱼IgM、NKCC1和Hsp70基因的表达
IgM, NKCC1, and Hsp 70 gene expression in juvenile Takifugu rubripes under acute low-salinity stress
摘要
Abstract
Takifugu rubripes is a popular food in China,Japan,and Korea because of its fresh meat and high nutritional value.In recent years,desalination marine fish farming has aroused interest,and T.rubripes is a marine model organism for studying low-salinity aquaculture because of its ability to tolerate a wide range of environmental salinities.This fish species has been used to explore the molecular mechanisms of osmoregulation in marine teleosts due to its small genome,and＞95％ of the genome has been sequenced.Studies have shown that T.rubripes can survive in hypo-osmotic conditions such as 10％-25％ saltwater,but the fish does not survive in freshwater.Therefore,to investigate the molecular mechanism of low-salinity tolerance in T.rubripes,the expression of three genes,including immunoglobulin M (IgM),Na-K-Cl cotransporter 1 (NKCC1),and heat shock protein 70 (Hsp70) was analyzed using real-time quantitative polymerase chain reaction (qRT-PCR) analysis of the gill and kidney from juvenile T.rubripes under low-salinity stress.T.rubripes juveniles were divided randomly into five groups and maintained in water with salinities of 32 (control) 16,12,8,and 4 for 72 h.The results show that all three genes were expressed in gill and kidney.IgM and Hsp70 expression levels were not different between the two tissues (P＞0.05),but NKCC1 expression level in the gill was significantly higher than that in the kidney (P＜0.05).IgM expression decreased initially and then increased in the gill,whereas it decreased initially,increased,and finally tended to stabilize in the kidney in fish under acute low-salinity stress.More notable differences were detected in the gill between the low-salinity and control groups compared with those in the kidney at the same time points.NKCC1 expression decreased in the gill but increased in the kidney under low-salinity stress,and NKCC1 expression levels in the gill were higher at all time points than that at 0 h.NKCC1 expression levels in the gill of fish in the low-salinity groups were significantly lower than those in the control group at 6 and 72 h (P＜0.05).Significant differences in gill Hsp70 expression levels were observed in the low-salinity groups at 3 h,6 h,24 h,and 72 h compared with those in the control.Changes in kidney Hsp70 expression began at 6 h.Taken together,these results reveal that IgM,NKCC1,and Hsp70 expression levels in the gill and kidney changed when juvenile T.rubripes were maintained in different salinities and for various durations.These results suggest that these three genes play an important role in the low-salinity tolerance of T.rubripes and provide basic data for clarifying the molecular mechanisms of osmoregulation in T.rubripes.关键词
红鳍东方鲀/实时荧光定量PCR/IgM/NKCC1/Hsp 70/急性低盐胁迫Key words
Takifugu rubripes/qRT-PCR/IgM/NKCC1/Hsp70/acute low-salinity stress分类
农业科技引用本文复制引用
孙梦蕾,吕绘倩,暴宁,司滨,陈飞,王莉苹,蒋洁兰..急性低盐胁迫下红鳍东方纯幼鱼IgM、NKCC1和Hsp70基因的表达[J].中国水产科学,2017,24(1):65-72,8.基金项目
辽宁省教育厅一般项目(L2015076) （L2015076）
国家海洋局公益性行业科研专项(201405003) （201405003）
大连海洋大学引进人才启动项目(HDYJ201501) （HDYJ201501）
农业部北方海水养殖重点实验室开放课题(2015-MSENC-KF-04). （2015-MSENC-KF-04）
