中国水产科学2017，Vol.24Issue(1)：180-189,10.DOI:10.3724/SP.J.1118.2017.16066
氨氮胁迫对中国明对虾血淋巴氨氮、尿素氮含量和抗氧化能力的影响
Effects of ambient ammonia-N exposure on hemolymph nitrogen metabolism and antioxidant capacity of Fenneropenaeus chinensis
摘要
Abstract
Chinese shrimp,Fenneropenaeus chinensis,is widely cultured in China for its high economic value.However,farming yields of F.chinensis have decreased in the past few decades.The decreases in yield has been attributed to disease,as environmental deterioration due to poor management has resulted in an increased incidence of stress-induced disease.Environmental stressors,such as ammonia,have a significant effect on the antioxidant system of shrimp.However,little information is available about the effects of changes in ambient ammonia-N on hemolymph nitrogen content and antioxidant capability of Chinese shrimp.Therefore,600 shrimps [initial body weight,(5.0±1.2) g] were divided randomly into five groups and exposed to water containing different concentrations of ambient ammonia-N (0 mg/L,2 mg/L,4 mg/L,6 mg/L,and 8 mg/L).Each group had six replicates of 20 shrimp each.Total antioxidant capacity (T-AOC),antisuperoxide anions,relative expression of catalase (CAT),peroxiredoxin (Prx),and caspase mRNA in hemocytes and hemolymph ammonia,and urea content were analyzed in hemolymph after exposing shrimp to different concentrations of ambient ammonia-N for 6 h,24 h,48 h,72 h,and 96 h.Shrimp cultured in filtered seawater were used as the control.The results showed that ammonia accumulated gradually in the hemolymph as exposure time increased.Hemolymph ammonia content of shrimp in the 8 mg/L group was the highest compared with that in the other groups and was 5.85 times higher than that in the control group.Hemolymph urea contents of shrimp in the ammonia-N exposed groups were higher than that in the control group after 6 h of ammonia-N exposure.The hemolymph urea of shrimp in the 6 mg/L group was the highest after 6 h and was 2.22-times higher than that in the control group.Hemolymph T-AOC and Prx mRNA expression in hemocytes of shrimp exposed to ambient ammonia-N increased initially and then decreased as exposure time increased.However,anti-superoxide anions in hemolymph and relative CAT and caspase mRNA expression levels in hemocytes increased,decreased,and then increased again as exposure time increased.The antioxidant capacity of shrimp was significantly higher after exposure to ammonia-N than that in the control.These findings demonstrate that the oxidation-antioxidant balance system in shrimp may be damaged after exposure to ammonia-N,resulting in upregulation of caspase mRNA expression.关键词
氨氮胁迫/中国明对虾/抗氧化能力/血淋巴Key words
Fenneropenaeus chinensis/ammonia-N exposure/antioxidant capacity/hemolymph分类
农业科技引用本文复制引用
王芸,李健,何玉英,段亚飞,张喆,李吉涛..氨氮胁迫对中国明对虾血淋巴氨氮、尿素氮含量和抗氧化能力的影响[J].中国水产科学,2017,24(1):180-189,10.基金项目
国家虾产业技术体系项目(CARS-47) （CARS-47）
国家自然科学基金项目(31172401) （31172401）
公益性行业(农业)科研专项(201103034). （农业）
