中国水产科学2017，Vol.24Issue(2)：220-230,11.DOI:10.3724/SP.J.1118.2017.16133
温度胁迫对皱纹盘鲍生理和生化活动的影响
Effects of temperature change on physiological and biochemical activities of Haliotis discus hannai Ino
摘要
Abstract
The effects of changes in temperature on physiological and biochemical activities of Haliotis discus hannai Ino were studied in the laboratory to better understand the thermal tolerance mechanisms of abalone. Aba-lone were transferred abruptly from a rearing temperature (15℃, control temperature) to 5℃, 10℃, 20℃, or 25℃. Other groups of abalone were acclimated to different experimental temperatures (5℃, 10℃, 20℃, and 25℃) by gradually increasing or decreasing seawater temperature (0.5℃/12 h). Then, oxygen consumption and ammonia excretion rates of H. discus hannai Ino were measured, the activities of superoxide dismutase (SOD), catalase, acid phosphatase and lysozyme (LSZ) in the digestive gland were determined, and Cu/Zn–SOD gene expression levels in hemocytes and muscle were determined. The results showed that oxygen consumption and ammonia-N excretion rates of H. discus hannai Ino increased gradually with an increase in water temperature and peaked at 20℃. The rates of oxygen consumption and ammonia-N excretion in the 25℃-acute temperature change treatment were significantly higher than those in the gradual treatment (P<0.01). Moreover, significant differences in the abalone O/N ratio were observed between the acute and gradual temperature change treatments at 5℃ and 10℃ (P<0.01). SOD, catalase, acid phosphatase, and LSZ activities increased significantly in abalone exposed to high temperature (25℃) for 72 h and reached the maximum at 3 h or 6 h (P<0.01). However, a significant decrease in LSZ activity was found in abalone in the 5℃-acute temperature change treatment and reached the lowest level after 9 h (P<0.01). Significantly up regulated Cu/Zn–SOD gene expression was also observed in hemocytes and muscle in abalone from the different temperature treatments (P<0.05). The physiological and biochemical activi-ties of H. discus hannai Ino were significantly affected by temperature. These data provide valuable insight into the possible mechanisms of abalone summer mortalities and will serve as a theoretical basis for healthy breeding of abalone.关键词
皱纹盘鲍/温度/生理和生化活动/Cu/Zn–SOD基因Key words
Haliotis discus hannai Ino/temperature/physiological and biochemical activities/Cu/Zn–SOD gene分类
农业科技引用本文复制引用
姜娓娓,方建光,李加琦,蒋增杰,毛玉泽,杜美荣,高振琨,陈琼琳..温度胁迫对皱纹盘鲍生理和生化活动的影响[J].中国水产科学,2017,24(2):220-230,11.基金项目
国家贝类产业技术体系项目(CARS-48). （CARS-48）
