中国水产科学2017，Vol.24Issue(2)：231-238,8.DOI:10.3724/SP.J.1118.2017.16150
刀鲚POMC基因的cDNA克隆及其应激应答
Cloning of POMC cDNA and the stress response in Coilia nasus
摘要
Abstract
The estuarine tapertail anchovy (Coilia nasus, junior synonymC. ectenes) is widely distributed in the Yangtze River, the coastal waters of China and Korea, and the Ariake Sound of Japan. It is a commercially impor-tant species because of its nutritive value and popularity. However, excess fishing and changes in aquatic ecology have almost caused extinction of this species in the middle reaches of the Yangtze River. In recent years, several studies have been performed on pond breeding and artificial propagation, and larval rearing techniques have been established. Thus, the threat toC. nasus resources has been alleviated. However, C. nasus is very sensitive to stress, which often results in death and huge economic losses. Little is known about the mechanism by which loading stress causes death in fish. Pro-opiomelanocortin (POMC) is synthesized in the corticotrophs and melano-trophs of the anterior and intermediate lobes of the pituitary, respectively, as well as in peptidergic neurons in the arcuate nucleus of the hypothalamus. It is post-translationally cleaved into peptide hormones, including adreno-corticotrophic hormone,β-endorphin,α-,β-, andγ-melanocyte stimulating hormone (MSH), N-POMC, andβ-lipotropin (β-LPH) in tissue- and cell dependent manners. These peptides exhibit different physiological func-tions, such as promoting ovarian maturation, lipolysis, inhibiting fat accumulation, regulating osmotic pressure, and reacting to stressors. We cloned the POMC gene inC. nasus and determined that the full-length cDNA is 1212 bp long, with a 699 bp open reading frame that encoded a 232 amino acid protein. The POMC protein is predicted to contain the typical POMC domains of a signal peptide sequence, the N-terminal region, and the adrenocortico-trophic hormone,β-LPH,α-MSH, CLIP,γ-LPH,β-MSH, andβ-EP domains. Gene expression patterns were de-tected in different tissues by quantitative reverse transcription-polymerasechain reaction.POMC was expressed strongly in the brain and relatively highly in the gill, kidney, and testis, whereas it was expressed at low levels in the liver, spleen, intestine, head kidney, muscle, and ovary.POMC was significantly down regulated after transport stress (P<0.05) and was significantly up regulated 4 h and 6 h after transport (P<0.05) in the 10‰ NaCl group (P<0.05). Moreover,POMC expression returned to the control level before stress. The POMC gene plays an im-portant role regulating fat metabolism and stress. Our results provide important clues for breeding and stress con-trol studies inC. nasus.关键词
刀鲚/POMC基因/应激应答/运输胁迫Key words
Coilia nasus/POMC gene/stress response/transport stress分类
农业科技引用本文复制引用
杜富宽,徐钢春,黎燕,练青平,徐跑..刀鲚POMC基因的cDNA克隆及其应激应答[J].中国水产科学,2017,24(2):231-238,8.基金项目
农业部淡水渔业健康养殖重点实验室开放课题(ZJK2014003) （ZJK2014003）
中国水产科学研究院淡水渔业研究中心中央级公益性科研院所基本科研业务费专项资金(2015JBFR06) （2015JBFR06）
江苏省科技支撑项目(BE2014307). （BE2014307）
