中国水产科学2017，Vol.24Issue(2)：239-247,9.DOI:10.3724/SP.J.1118.2017.16136
三疣梭子蟹卵巢发育过程中雌二醇的免疫定位和变化
Immunolocalization and changes in 17 β-estradiol in Portunus tritu-berculatus during ovarian development
摘要
Abstract
17β-Estradiol (E2) is among the most important sex steroid hormones during ovarian development and vitellogenesis in crustaceans. In this study, an immunofluorescence assay was used to identify the immunopositive distribution and variations in E2 in the ovary, hepatopancreas, thoracic ganglia, brain ganglion, and the mandibular organ during ovarian development in the swimming crab,Portunus trituberculatus. The results showed that E2 was mainly distributed in the follicular cells and the cytoplasm of late-stage oocytes (Ⅱ–Ⅴ). Follicular cells stained strongly positive for E2 at all ovarian stages; the immunoreactivities of E2 in the oocyte cytoplasm and nucleus trended as "low-high-low", and maximum positive staining appeared during ovarian stage Ⅳ and stages Ⅱ–Ⅲ, respectively. In the hepatopancreas, E2-positive staining was mainly distributed in F cells and R nuclei, and E2 immunoreactivity in the R nucleus tended to decrease from stages Ⅲ to Ⅳ. However, strong positive E2 immuno-reactivity was always detected in F cells at all ovarian stages. E2 was mainly distributed in neurons, the nerve medulla of thoracic ganglia, and cell nuclei of cerebral ganglion nerve cells during the early stages of ovarian de-velopment (Ⅰ–Ⅱ). E2 immunore activity in the nerve medulla of the thoracic ganglion was moderately positive, where as neurons were always weakly positive. Strong positive E2 staining was always distributed in the nuclei of mandibular gland cells and the cytoplasm around mandibular gland cellnuclei. These results suggest that E2 is widely distributed in the ovary, hepatopancreas, thoracic ganglion, cerebral ganglion, and mandibular gland of P. trituberculatus, and a correlation was detected between E2 immunoreactivity and ovarian developmental stage. E2 may act through multiple target organs to regulate ovarian development in P. trituberculatus.关键词
三疣梭子蟹/卵巢发育/雌二醇/免疫定位/作用靶点Key words
Portunus trituberculatus/ovarian development/estradiol/immunolocalization/action target分类
农业科技引用本文复制引用
柳梅梅,吴旭干,潘杰,成永旭..三疣梭子蟹卵巢发育过程中雌二醇的免疫定位和变化[J].中国水产科学,2017,24(2):239-247,9.基金项目
国家自然科学基金项目(41276158) （41276158）
上海市自然科学基金项目(12ZR1413000) （12ZR1413000）
上海市科技兴农重点攻关项目[(沪农科攻字(2013)第6-3号)] （沪农科攻字(2013）
上海市科技兴农推广项目[沪农科攻字(2016)第1-1-8号] （2016）
上海市高峰学科建设项目(2012-62-0908) （2012-62-0908）
浙江省东海海水养殖产业升级协同创新中心项目. （）
